LEICA TCS SP CONFOCAL MICROSCOPE

本申请的目的是获得一个更新的共聚焦显微镜的牙科显微镜核心设施的贝勒学院。 该设施目前拥有一个子午线洞察共聚焦显微镜，蔡司epifluorescent直立显微镜和尼康直立显微镜与CCD相机和变形软件。 需要资金购买一个Leica TICS SP共焦图像分析工作站，配有倒置和直立显微镜。 扫描仪将根据项目需要移动到适当的显微镜。 该软件包括3D和生理学程序，因为一些研究人员计划获得用活性染料、钙和pH指示剂染色的活组织的延时记录。 目前，生物医学科学系已经为显微镜分配了空间。我们获得了贝勒口腔健康基金会的机构支持，以获得匹配资金。 已经确定了八个主要和六个次要研究小组，他们需要新设备进行广泛的项目。 研究人员跨越了从年轻研究人员到高级科学家的许多年龄组。大多数参与者目前有PHS赠款支持或赠款待定。 他们都期待着在核心设施中使用该设备来加强和扩展他们的研究。 我们目前的机器只有6年的历史，但是，整个系统已经过时，因为公司被出售，显微镜部门被淘汰。 这个系统需要修理，如果能找到零件，它们非常昂贵。 此外，由于它只配备了氩激光器和CCD相机，因此可以查看的样品类型有限。

The purpose of this application is to acquire an updated confocal microscope for the established Baylor College of Dentistry microscope core facility. The facility currently houses a Meridian Insight confocal microscope, a Zeiss epifluorescent upright microscope and a Nikon upright microscope with a CCD camera and Metamorph software. Funds are requested to purchase a Leica TICS SP confocal image analysis work station with both inverted and upright microscopes. The scanner will be moved to the appropriate microscope as needed by the projects. The software includes 3-D and physiology programs as several researchers plan to obtain time lapse recordings of live tissue stained with vital dyes, calcium and pH indicators. Currently the Biomedical Sciences Department has allocated space for the microscope. We have obtained institutional support from the Baylor Oral Health Foundation for matching funds. Eight primary and six secondary research groups have been identified that need the new equipment for a broad range of projects. The researchers span many age groups from young investigators to senior scientists. Most participants have current PHS grant support or have grants pending. They are all looking forward to enhancing and extending their research with this equipment in the core facility. Our current machine is only 6 years old, however, the whole system is obsolete as the company was sold and the microscope division was phased out. The system needs repairs and if the parts can be found they are very expensive. In addition, it is limited in the types of specimens that can be viewed as it is equipped with only an argon laser and a CCD camera.