LEICA TCS SP CONFOCAL MICROSCOPE

徕卡 TCS SP 共焦显微镜

• 批准号: 6051659
• 负责人: KATHY Kay SVOBODA
• 金额: $ 18.75万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6051659
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

The purpose of this application is to acquire an updated confocal microscope for the established Baylor College of Dentistry microscope core facility. The facility currently houses a Meridian Insight confocal microscope, a Zeiss epifluorescent upright microscope and a Nikon upright microscope with a CCD camera and Metamorph software. Funds are requested to purchase a Leica TICS SP confocal image analysis work station with both inverted and upright microscopes. The scanner will be moved to the appropriate microscope as needed by the projects. The software includes 3-D and physiology programs as several researchers plan to obtain time lapse recordings of live tissue stained with vital dyes, calcium and pH indicators. Currently the Biomedical Sciences Department has allocated space for the microscope. We have obtained institutional support from the Baylor Oral Health Foundation for matching funds. Eight primary and six secondary research groups have been identified that need the new equipment for a broad range of projects. The researchers span many age groups from young investigators to senior scientists. Most participants have current PHS grant support or have grants pending. They are all looking forward to enhancing and extending their research with this equipment in the core facility. Our current machine is only 6 years old, however, the whole system is obsolete as the company was sold and the microscope division was phased out. The system needs repairs and if the parts can be found they are very expensive. In addition, it is limited in the types of specimens that can be viewed as it is equipped with only an argon laser and a CCD camera.

本申请的目的是为已建立的贝勒牙科学院显微镜核心设施获得最新的共焦显微镜。该设施目前拥有一台Meridian Insight共聚焦显微镜、一台蔡司荧光直立式显微镜和一台尼康直立式显微镜，该显微镜带有CCD摄像机和变形软件。需要资金购买徕卡公司SP共聚焦图像分析工作站，该工作站既有倒置显微镜，也有立式显微镜。根据项目的需要，扫描仪将被移至适当的显微镜。该软件包括3-D和生理学程序，因为几名研究人员计划获得用生命染料、钙和pH指示剂染色的活组织的延时记录。目前，生物医学科学系已经为显微镜分配了空间。我们已经从贝勒口腔健康基金会获得了机构支持，以获得匹配资金。已确定8个初级研究小组和6个次级研究小组需要为广泛的项目配备新设备。研究人员涵盖了从年轻研究人员到资深科学家的许多年龄段。大多数参与者都有目前的小灵通补助金支持，或者还有未决的补助金。他们都期待着在核心设施中利用这一设备加强和扩大他们的研究。我们目前的机器只有6年的历史，然而，整个系统已经过时，因为公司被出售，显微镜部门被逐步淘汰。这个系统需要维修，如果能找到零件，它们就会非常昂贵。此外，由于它只配备了一台Ar激光器和一台ccd摄像机，因此可以观察的标本类型有限。