LEICA TCS SP CONFOCAL MICROSCOPE

此应用程序的目的是为已建立的贝勒牙科学院显微镜核心设施获取更新的共焦显微镜。 该设施目前拥有一台 Meridian Insight 共焦显微镜、一台蔡司落射荧光正置显微镜和一台配备 CCD 相机和 Metamorph 软件的尼康正置显微镜。 需要资金购买 Leica TICS SP 共焦图像分析工作站，配有倒置和正置显微镜。 扫描仪将根据项目需要移动到适当的显微镜。 该软件包括 3-D 和生理学程序，因为一些研究人员计划获得用活体染料、钙和 pH 指示剂染色的活组织的延时记录。 目前生物医学科学系已为显微镜分配了空间。我们获得了贝勒口腔健康基金会的配套资金机构支持。 已确定八个初级研究小组和六个二级研究小组需要新设备来开展广泛的项目。 研究人员跨越许多年龄段，从年轻的研究人员到资深的科学家。大多数参与者目前都有 PHS 拨款支持或正在等待拨款。 他们都期待着利用核心设施中的设备来加强和扩展他们的研究。 我们当前的机器只有 6 年的历史，然而，随着公司被出售且显微镜部门被逐步淘汰，整个系统已经过时。 该系统需要维修，如果能找到零件，价格会非常昂贵。 此外，由于仅配备了氩激光器和CCD相机，因此可观察的标本类型受到限制。

The purpose of this application is to acquire an updated confocal microscope for the established Baylor College of Dentistry microscope core facility. The facility currently houses a Meridian Insight confocal microscope, a Zeiss epifluorescent upright microscope and a Nikon upright microscope with a CCD camera and Metamorph software. Funds are requested to purchase a Leica TICS SP confocal image analysis work station with both inverted and upright microscopes. The scanner will be moved to the appropriate microscope as needed by the projects. The software includes 3-D and physiology programs as several researchers plan to obtain time lapse recordings of live tissue stained with vital dyes, calcium and pH indicators. Currently the Biomedical Sciences Department has allocated space for the microscope. We have obtained institutional support from the Baylor Oral Health Foundation for matching funds. Eight primary and six secondary research groups have been identified that need the new equipment for a broad range of projects. The researchers span many age groups from young investigators to senior scientists. Most participants have current PHS grant support or have grants pending. They are all looking forward to enhancing and extending their research with this equipment in the core facility. Our current machine is only 6 years old, however, the whole system is obsolete as the company was sold and the microscope division was phased out. The system needs repairs and if the parts can be found they are very expensive. In addition, it is limited in the types of specimens that can be viewed as it is equipped with only an argon laser and a CCD camera.