CORE--CELL DISAGGREGATION AND CULTURE

子项目摘要不可用。 文章摘要： 我们的计划重点是心脏节律和心律失常的决定因素 在年轻的时候。 我们的假设是 年轻人的电生理功能受自主神经的影响， 神经系统和心脏的电生理特性 与成年人有很大的不同，这需要我们深入研究 了解我们是否要预防和治疗心脏跳动紊乱。 因此，我们打算研究新生、幼龄和成年动物。 我们 方法是多方面的，在电生理，生物物理， 生物化学、药理学和细胞培养技术用于 完整动物、分离组织、单细胞和亚细胞研究 件. 尽管胚胎发生一般可以被认为是 包括冲动起始和/或传导的异常， 在这五年期间，我们的目标是集中力量推动 以及它与复极的关系。 该计划中的项目 强调研究完整动物的电生理特性， 离体组织，离子决定因素的冲动启动研究中， 上述模型（项目A）和单个肌细胞和培养细胞，以及 受体-效应器偶联。 核心提供支持服务，细胞分解和组织 文化 虽然我们的整体方法包括成人作为一个主要的 参考人群，最密集的调查领域是 以新生儿和年轻人为中心。 这些研究的意义在于 他们是面向年轻人的， 哪一种节律和心律失常在它们的起源和自主神经方面真正不同 成年人的调节。 利用所获得的信息，我们不仅 将提高我们对负责正常的机制的理解 但可以设计和寻求新的手段， 预防和治疗心律失常。

SUBPROJECT ABSTRACT NOT AVAILABLE. PARENT ABSTRACT: Our program focuses on the determinants of cardiac rhythm and arrhythmias in the young. Our hypothesis is that the expression of electrophysiologic function in the young is influenced by the autonomic nervous system and the electrophysiologic properties of the heart in ways that differ importantly from the adult, and that require our in depth understanding if we are to prevent and treat disorders of the heart beat. Hence, we intend to study the neonatal, young and adult animal. Our approach is multifaceted in that electrophysiological, biophysical, biochemical, pharmacological, and cell culture techniques are used in studies of intact animals, isolated tissues, single cells and subcellular components. Although arrhythmogenesis in general can be thought of as encompassing abnormalities of impulse initiation and/or conduction, our intent in this five-year period is to concentrate on impulse initiation and its relationship to repolarization. The projects in the Program stress the study of electrophysiologic properties of intact animals and isolated tissues, ionic determinants of impulse initiation studied in the above models (Project A) and in single myocytes and cultured cells, and receptor-effector coupling. The Cores provide support services, and cell disaggregation and tissue culture. Although our overall approach includes the adult as a major reference population, the most intensive area of investigation is centered on the neonate and young. The significance of these studies is that they are geared to the young age group as a unique population in which rhythm and arrhythmias truly differ in their genesis and autonomic modulation from the adult. Using the information obtained we not only shall improve our understanding of the mechanisms responsible for normal rhythm and arrhythmias but can design and seek new means for the prevention and treatment of arrhythmias.