NOVEL TOOLS FOR EVALUATING INTESTINAL DYSFUNCTION IN CHILDREN AND ADULTS WITH MALNUTRITION DISORDERS

评估患有营养不良疾病的儿童和成人肠功能障碍的新工具

• 批准号: MR/V012452/1
• 负责人: Paul Kelly
• 金额: $ 374.13万
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FV012452%2F1
• 关键词: NOVEL; TOOLS; EVALUATING; INTESTINAL; DYSFUNCTION

Mortality in children in Africa with SAM is still far higher than commonly appreciated, usually over 10% even for the 2-3 weeks that a child is in hospital if there is any sort of complication. It continues to occur even after discharge from hospital care. Several clinical trials are under way to try and find interventions to reduce this mortality, but they are hampered by the inadequacy of the tools at our disposable for measuring processes like impaired digestion, impaired absorption, failure of gut immunity, and disturbances of the balance of bacteria in the intestine. Even in highly refined health care systems these processes are difficult to assess, but in Africa this is a very tough obstacle to evaluating novel interventions. The study we propose will use stable (non-radioactive) isotope techniques to measure digestion and absorption, fluorescent tracers to measure leakiness of the lining of the gut, advanced immunology to study responses to molecules derived from bacteria in the gut, genomic sequencing to study the balance of bacteria in the gut, and advanced spectroscopy to analyse the function of those gut bacteria. In the process we will learn a great deal about children with severe acute malnutrition, and will generate new tools for analysing gut function. These tools may then, in turn, be useful for studying rare disorders of gut function in children and adults even in well-resourced countries, conditions which often require complex nutritional support and in which it is still, sadly, very hard to evaluate response to therapy.

非洲SAM儿童的死亡率仍然远远高于通常的估计，如果有任何并发症，即使在住院的2-3周内，死亡率也通常超过10%。甚至在出院后仍继续发生。一些临床试验正在进行中，试图找到降低这种死亡率的干预措施，但由于我们一次性使用的工具不足，无法测量消化受损，吸收受损，肠道免疫功能衰竭和肠道细菌平衡紊乱等过程，这些试验受到阻碍。即使在高度完善的卫生保健系统中，这些过程也很难评估，但在非洲，这是评估新干预措施的一个非常坚韧的障碍。我们提出的研究将使用稳定（非放射性）同位素技术来测量消化和吸收，荧光示踪剂来测量肠道衬里的泄漏，先进的免疫学来研究对肠道细菌分子的反应，基因组测序来研究肠道细菌的平衡，以及先进的光谱学来分析这些肠道细菌的功能。在这个过程中，我们将了解大量关于严重急性营养不良儿童的信息，并将产生分析肠道功能的新工具。反过来，这些工具可能有助于研究儿童和成人的罕见肠道功能障碍，即使在资源充足的国家，这些疾病通常需要复杂的营养支持，并且遗憾的是，仍然很难评估对治疗的反应。

项目成果

A compact fluorescence sensor for low-cost non-invasive monitoring of gut permeability in undernutrition

一种紧凑型荧光传感器，用于低成本、无创监测营养不良情况下的肠道通透性

• DOI: 10.1117/12.2649030
• 发表时间: 2023
• 作者: Monfort Sánchez E

Understanding the role of the gut in undernutrition: what can technology tell us?

• DOI: 10.1136/gutjnl-2020-323609
• 发表时间: 2021-06-08
• 期刊: Gut
• 影响因子: 24.5
• 作者: Thompson AJ;Bourke CD;Robertson RC;Shivakumar N;Edwards CA;Preston T;Holmes E;Kelly P;Frost G;Morrison DJ;HUNGer Consortium
• 通讯作者: HUNGer Consortium