DELAYED EXTRACTION UV & INFRARED WAVELENGTH MALDI MS

延迟萃取紫外线

• 批准号: 6118338
• 负责人: WENZHU ZHANG
• 金额: $ 1.71万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-10 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118338
• 关键词: biomedical resource; proteins; spectrometry; technology /technique

We have designed and constructed a robust and inexpensive high voltage pulsing system for carrying out delayed-extraction MALDI time-of-flight MS measurements. We have used this system to run both of our linear time-of-flight instruments either in continuous-extraction (CE) or delayed-extraction (DE) modes. Comparison of UV- and IR-MALDI using CE and DE has led to a number of interesting discoveries.. Thus, for example, we have found that IR-MALDI produces demonstrably cooler protein ions than UV-MALDI and that the tails on both IR- and UV-MALDI spectra originate from multiple association of Na cations with the protein. The results have been reported W. Zhang, S. Niu, B.T. Chait "Exploring Infrared Wavelength Matrix-Assisted Laser Desorption/Ionization of Proteins with Delayed Time-of-Flight Mass Spectrometry" J. Am. Soc. Mass Spectrom. 9 (1988) 879-884.

我们设计并建造了一个坚固而廉价的高 用于进行延迟提取MALDI的电压脉冲系统 飞行时间MS测量。 我们用这个系统来运行 我们的线性飞行时间仪器无论是在 连续提取（CE）或延迟提取（DE）模式。 使用CE和DE的UV-和IR-MALDI的比较导致了一些 有趣的发现 例如，我们发现， IR-MALDI比UV-MALDI产生明显更冷的蛋白质离子， IR和UV-MALDI光谱上的尾部都来自于 Na阳离子与蛋白质的多重缔合。 收效 据报道，W。Zhang，S. Niu，B.T. Chait“探索红外线 波长矩阵辅助激光解吸/电离蛋白质 用Delayed Time-of-Flight Mass Spectrometry”J. Am.社会质量 幽灵 9（1988）879-884。