DELAYED EXTRACTION UV & INFRARED WAVELENGTH MALDI MS

延迟萃取紫外线

• 批准号: 6307578
• 负责人: WENZHU ZHANG
• 金额: $ 0.82万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6307578
• 关键词: biomedical equipment development; biomedical resource; proteins; spectrometry; technology /technique

We have designed and constructed a robust and inexpensive high voltage pulsing system for carrying out delayed-extraction MALDI time-of-flight MS measurements. We have used this system to run both of our linear time-of-flight instruments either in continuous-extraction (CE) or delayed-extraction (DE) modes. Comparison of UV- and IR-MALDI using CE and DE has led to a number of interesting discoveries which we will report in a manuscript in preparation. Thus, for example, we have found that IR-MALDI produces demonstrably cooler protein ions than UV-MALDI and that the tails on both IR- and UV-MALDI spectra originate from multiple association of Na cations with the protein.

我们已经设计并建造了一个坚固而廉价的高