NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS

用于紧急输血的人造血：由 计算机辅助分子模拟，我们设计和合成了新的 用于交联无细胞血红蛋白的多官能团有机试剂。 这些修饰后的血红蛋白有可能成为 用于紧急输血的全血，不需要打字或 交叉搭配，不含细菌或病毒，可作为干燥剂储存 无限期的粉末状。其交联度为 预计会防止肾脏在其他方面的快速消除 无细胞血红蛋白，同时也减少了后来的氧气 亲和力，以便提供足够的氧气和二氧化碳运输 从肺到组织，反之亦然。为实现上述目标， 我们需要在32,000-33,000范围内进行高质量分析 由MALDI-MS提供。四个血红蛋白亚基，？1，？1，？2，？2， 重约16,000道尔顿。当交叉连接时，它们形成一种 二聚体？32000道尔顿。因此，MALDI-MS将使我们能够 方便地a)分析交联型图案，b)在 结合胰酶消化，绘制出交联区 已经发生了。

Artificial Blood for Emergency Transfusions: Guided by computer-assisted molecular modeling, we design and synthesize novel polyfunctional organic reagents to cross-link cell-free hemoglobin. These modified hemoglobins will potentially serve as substitutes for whole blood for emergency transfusions, and would require no typing or cross-matching, be free of bacteria or viruses, and storable as a dry powder for indefinite periods of time. The cross-linking is anticipated to prevent the otherwise rapid renal elimination of cell-free hemoglobins, while also decreasing the latters oxygen affinity, so as to afford adequate oxygen and carbon dioxide transport from lungs to tissues and vice versa. To accomplish the above goal, we need an access to high mass analysis in the range of 32,000-33,000 by MALDI-MS. Each of the four hemoglobin subunits, ?1, ?1, ?2, ?2, weighs approximately 16,000 Daltons. When cross-linked, they form a dimer of ?32,000 Daltons. Therefore, MALDI-MS would enable us to conveniently a) analyze the cross-linking patterns, and b) in conjunction with tryptic digestion, map regions where cross-linking has occurred.