NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
批准号:
6258823
负责人:
Ramachandra S Hosmane
金额:
$0.02万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-06-01 至 1999-11-30
中文摘要
用于紧急输血的人造血:由
计算机辅助分子模拟,我们设计和合成了新的
用于交联无细胞血红蛋白的多官能团有机试剂。
这些修饰后的血红蛋白有可能成为
用于紧急输血的全血,不需要打字或
交叉搭配,不含细菌或病毒,可作为干燥剂储存
无限期的粉末状。其交联度为
预计会防止肾脏在其他方面的快速消除
无细胞血红蛋白,同时也减少了后来的氧气
亲和力,以便提供足够的氧气和二氧化碳运输
从肺到组织,反之亦然。为实现上述目标,
我们需要在32,000-33,000范围内进行高质量分析
由MALDI-MS提供。四个血红蛋白亚基,?1,?1,?2,?2,
重约16,000道尔顿。当交叉连接时,它们形成一种
二聚体?32000道尔顿。因此,MALDI-MS将使我们能够
方便地a)分析交联型图案,b)在
结合胰酶消化,绘制出交联区
已经发生了。
英文摘要
Artificial Blood for Emergency Transfusions: Guided by
computer-assisted molecular modeling, we design and synthesize novel
polyfunctional organic reagents to cross-link cell-free hemoglobin.
These modified hemoglobins will potentially serve as substitutes for
whole blood for emergency transfusions, and would require no typing or
cross-matching, be free of bacteria or viruses, and storable as a dry
powder for indefinite periods of time. The cross-linking is
anticipated to prevent the otherwise rapid renal elimination of
cell-free hemoglobins, while also decreasing the latters oxygen
affinity, so as to afford adequate oxygen and carbon dioxide transport
from lungs to tissues and vice versa. To accomplish the above goal,
we need an access to high mass analysis in the range of 32,000-33,000
by MALDI-MS. Each of the four hemoglobin subunits, ?1, ?1, ?2, ?2,
weighs approximately 16,000 Daltons. When cross-linked, they form a
dimer of ?32,000 Daltons. Therefore, MALDI-MS would enable us to
conveniently a) analyze the cross-linking patterns, and b) in
conjunction with tryptic digestion, map regions where cross-linking
has occurred.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Inhibition of HCV as an Opportunistic HIV Co-infection
-
批准号:7554954
-
项目类别:
-
资助金额:$34.29万
-
财政年份:2008
-
负责人:Ramachandra S Hosmane
-
依托单位:
Mechanistic Studies of Anti-HIV Activity of a Novel Ring-Expanded Nucleoside
-
批准号:7167872
-
项目类别:
-
资助金额:$22.69万
-
财政年份:2006
-
负责人:Ramachandra S Hosmane
-
依托单位:
Mechanistic Studies of Anti-HIV Activity of a Novel Ring-Expanded Nucleoside
-
批准号:7274009
-
项目类别:
-
资助金额:$4.85万
-
财政年份:2006
-
负责人:Ramachandra S Hosmane
-
依托单位:
Mechanistic Studies of Anti-HIV Activity of a Novel Ring-Expanded Nucleoside
-
批准号:7268036
-
项目类别:
-
资助金额:$23.69万
-
财政年份:2006
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT & SLIM NUCLEOSIDE & NUCLEOTIDE: ANTICANCER & ANTIVIRAL AGENT AIDS
-
批准号:6248390
-
项目类别:
-
资助金额:$0.46万
-
财政年份:1997
-
负责人:Ramachandra S Hosmane
-
依托单位:
CHAIN TERMINATORS IN HIV REVERSE TRANSCRIPTION
-
批准号:6258822
-
项目类别:
-
资助金额:$0.01万
-
财政年份:1997
-
负责人:Ramachandra S Hosmane
-
依托单位:
NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
-
批准号:6248391
-
项目类别:
-
资助金额:$0.46万
-
财政年份:1997
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2827772
-
项目类别:
-
资助金额:$1.44万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
'Fat' Nucleosides and Nucleotides
-
批准号:6861031
-
项目类别:
-
资助金额:$33.54万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
'Fat' Nucleosides and Nucleotides
-
批准号:6726210
-
项目类别:
-
资助金额:$28.71万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2114811
-
项目类别:
-
资助金额:$17.57万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
'Fat' Nucleosides and Nucleotides
-
批准号:6544564
-
项目类别:
-
资助金额:$14.36万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2733233
-
项目类别:
-
资助金额:$22.33万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2550396
-
项目类别:
-
资助金额:$0.32万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
'Fat' Nucleosides and Nucleotides
-
批准号:6896037
-
项目类别:
-
资助金额:$4.7万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2443252
-
项目类别:
-
资助金额:$23.1万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
FAT AND SLIM NUCLEOSIDES AND NUCLEOTIDES
-
批准号:2653186
-
项目类别:
-
资助金额:$1.44万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
'Fat' Nucleosides and Nucleotides
-
批准号:6665125
-
项目类别:
-
资助金额:$28.72万
-
财政年份:1996
-
负责人:Ramachandra S Hosmane
-
依托单位:
NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
-
批准号:2224702
-
项目类别:
-
资助金额:$12.51万
-
财政年份:1994
-
负责人:Ramachandra S Hosmane
-
依托单位:
NOVEL ORGANIC REAGENTS FOR BIOMEDICAL APPLICATIONS
-
批准号:2224703
-
项目类别:
-
资助金额:$12.08万
-
财政年份:1994
-
负责人:Ramachandra S Hosmane
-
依托单位:
海外基金