CHAIN TERMINATORS IN HIV REVERSE TRANSCRIPTION

HIV 逆转录中的链终止子

• 批准号: 6258822
• 负责人: Ramachandra S Hosmane
• 金额: $ 0.01万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6258822
• 关键词: biological products; biomedical resource; proteins; technology /technique development

Fat and Slim Nucleosides and Nucleotides as Potential Aticancer and Antiviral Agents: These novel ring-expanded (fat) and ring-contracted (slim) nucleoside analogues, designed and synthesized in this lab, are potential chain-terinators of nucleic acid synthesis when incorporated into a tumor or viral DNA/RNA during transcription (or reverse transcription in case of retroviruses including HIV that causes AIDS). Mode of chain termination, and hence the viral or tumor replication, is believed to be the base-mispairing accompanied by considerable deviation of the base-sugar bond from the natural array. In addition, because of their unique structural, spatial, and conformational characteristics and constraints, fat and slim nucleosides/-tides are excellent probes for nucleic acid metabolism, structure, and function. While we use EI/CI/FAB Mass Spectrometry for routine structural analyses of small molecules, the MALDI TOF mass spectrometer is necessary for the molecular weight determination and purity of oligonucleotides derived from or incorporated with fat or slim nucleotides.

脂肪 和 苗条 核苷和核苷酸作为潜在的抗癌剂 和抗病毒剂：这些新的环扩展（脂肪）和 环收缩（苗条核苷类似物，设计和合成 在这个实验中，是核酸合成的潜在链终止子 当在转录过程中掺入肿瘤或病毒DNA/RNA时 (or在逆转录病毒包括HIV的情况下， 导致艾滋病）。 链终止模式，因此病毒或肿瘤 复制，被认为是碱基错配伴随着 基糖键与天然排列的相当大的偏差。 此外，由于其独特的结构、空间和 构象特征和限制， 脂肪 和 苗条 核苷/核苷酸是核酸代谢的极好探针， 结构和功能。 虽然我们使用EI/CI/FAB质谱法 小分子的常规结构分析，MALDI TOF质量 分子量测定需要光谱仪， 衍生自或掺入的寡核苷酸的纯度 脂肪 或 苗条 个核苷酸