NATIONAL RESOURCE FOR APLYSIA

这项建议描述了建立一个国家资源用于 Aplysia，其总体目标是提供始终如一的高质量 养殖的海兔(及其养殖的红藻) 食用鱼(Gracilaria sp.)给NIH赞助的研究人员。答：加州是一个 重要的非脊椎动物(异型软体动物)模型系统 与健康相关的研究，在行为和神经生理学方面 学习。我们将生产用于研究的动物，并将进行基础 研究旨在探索新的模式用途并改善 资源。有四个子项目/具体目标。1.制作-我们 将使我们1994年的预计产量比去年增加约225% 这笔赠款的有效期，并将制作所有生命阶段的标本 与现场采集的标本相比，价格具有竞争力。2. 遗传学-我们将进行种群遗传学研究，以确定 在我们的孵化场群体中存在的遗传变异量 与自然种群相比。这种遗传信息的使用 将为NIH赞助的调查人员提供杂合性数据 用于孵化种群，并具有遗传标记 想要的/不想要的特征。3.发育神经生理学--这个 该项目将研究神经元兴奋性的调节 影响生殖发育的。一种单电池电压钳 这项技术将被应用于研究中国经济的发展 调制刺激作用于离子电流的能力 调节刺激能力发展的实验研究 作用于神经分泌袋细胞的离子电流。这方面的发展 调节能力是性成熟不可或缺的一部分。这 子项目将进一步描述未成熟A. Calfornica，可能导致新模型的使用，并将产生 对孵化场成熟和繁殖策略有用的信息。4. 动物健康监测-我们将根据以下情况建立监测计划 筛选水质和动物健康参数以确保快速 检测并完整记录可能发生的任何疾病过程 发生在孵化场动物的任何发育阶段。任何 将对观察到的疾病症状和疑似病原体进行调查 并采取适当的控制措施。

This proposal describes the establishment of a National Resource for Aplysia, whose overall goal is to provide consistently high quality cultured sea hares aplysia californica (and their cultured red algae food Gracilaria sp.) To NIH sponsored researchers. A. Californica is an important non- vertebrate (opisthobranch mollusc) model system for health related research, in the neurophysiology of behavior and learning. We will produce animals for research, and will conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-project/specific aims. 1. Production- We will increase our projected 1994 production by approximately 225% over the tenure of this grant and will make specimens from all life stages available at a price competitive with field-collected specimens. 2. Genetics- We will perform population genetic studies to characterize the amount of genetic variation present in our hatchery population in comparison to natural populations. The use of this genetic information will be to provide NIH-sponsored investigators with heterozygosity data for the hatchery population and with genetic markers for desirable/undesirable traits. 3. Developmental Neurophysiology-This project will investigate the regulation of neuronal excitability affecting reproductive development. The single cell voltage clamp technique will be applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in neurosecretory bag cells. Development of this modulatory capability is an integral part of sexual maturation. This sub-project will further characterize the neurophysiology of immature A. Californica, potentially leading to a new model use, and will yield information useful to hatchery maturation and breeding strategies. 4. Animal Health Monitoring-We will establish a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animal at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied.