Investigating the role of perivascular mesenchymal stem cells in macular fibrosis secondary to neovascular age-related macular degeneration

研究血管周围间充质干细胞在新生血管性年龄相关性黄斑变性继发的黄斑纤维化中的作用

基本信息

  • 批准号:
    MR/W004682/1
  • 负责人:
  • 金额:
    $ 95.29万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2022
  • 资助国家:
    英国
  • 起止时间:
    2022 至 无数据
  • 项目状态:
    未结题

项目摘要

Age-related macular degeneration (AMD) is a disease that affects the macula, the central part of the retina at the back of the eye, causing progressive loss of central vision in the elderly. Globally, 200 million people are affected by AMD and this number is expected to increase to 300 million by 2040. In the developed world, AMD is the most common cause of blindness in the elderly. AMD has two advanced forms: wet (also known as neovascular AMD) and dry (also known as geographic atrophy); the former accounts for ~80% of AMD-related visual impairment. Wet AMD occurs when diseased blood vessels grow into the macula, causing fluid leakage and bleeding that impairs vision. It is currently treated with injections of VEGF inhibitor (e.g. Lucentis or Avastin) into the eye, which reduces the growth of blood vessels. Although such treatment can stabilise or even improve visual function, 50% of treated eyes eventually develop fibrosis (scarring) in the macula. Unlike scarring of the skin, which heals the wound, scarring in the macula, instead reduces the efficacy of VEGF inhibitors. The fibrovascular membranes that emerge from diseased blood vessels eventually destroy the entire macula. Currently, there are no medications to prevent or treat this condition due to a poor understanding of the disease mechanism. New blood vessels in wet AMD develop into fibrovascular scar when cells, called myofibroblasts, infiltrate and accumulate in the macula. Myofibroblasts produce excessive amounts of scar-forming extracellular matrix such as collagens and fibronectins. Myofibroblasts are absent from the healthy retina, including the macula, and we do not yet know where they come from and how they are activated in wet AMD. This knowledge is crucial for developing therapeutic interventions to wet AMD and macular fibrosis. Recently, perivascular mesenchymal stem cells (pMSC), a unique type of cell residing around blood vessels were identified as a major source of myofibroblasts in injury-induced scars in multiple organs, including the lung, kidney, heart, and skin. These pMSCs are known to safeguard blood vessels and maintain their integrity. During injury, they detach from blood vessel walls and travel to the site of damage, where they participate in tissue repair and regeneration. When the injury persists or when injury-mediated inflammation does not resolve promptly, these pMSCs expand and become myofibroblasts leading to organ fibrosis. We have found that within the eye, the retina and choroid also contain a network of pMSCs. In an experimental model of wet AMD-mediated retinal fibrosis, over 60% of myofibroblast originated from pMSCs, and genetic deletion of pMSCs reduced retinal fibrosis. Our aim is to understand why pMSCs become myofibroblasts in wet AMD and how we might prevent or reverse this process. We will use advanced genomic techniques to uncover the gene expression profile of individual pMSCs within the diseased tissue at different stages of retinal fibrosis. This will inform us of which pMSC subtypes give rise to myofibroblasts and the pathways that control this process. We will verify these pathways in pMSC cultures derived from the retina, choroid, and retinal scar tissue. We will then target these pathways with pharmacological approaches to prevent or reverse the differentiation of pMSCs into myofibroblasts using in vitro and in vivo models of retinal fibrosis. Our results will inform further research and enhance our understanding of retinal repair and fibrosis in wet AMD and other sight-threatening diseases caused by abnormal tissue repair (e.g. proliferative diabetic retinopathy [PDR] and proliferative vitreoretinopathy [PVR]). Ultimately, our research will aim in developing effective treatments for retinal fibrosis.
视网膜相关性黄斑变性(AMD)是一种影响黄斑的疾病,黄斑是眼睛后部视网膜的中心部分,导致老年人中心视力逐渐丧失。在全球范围内,有2亿人受到AMD的影响,预计到2040年这一数字将增加到3亿。在发达国家,AMD是老年人失明的最常见原因。AMD有两种高级形式:湿性(也称为新生血管性AMD)和干性(也称为地图状萎缩);前者占AMD相关视力损害的约80%。湿性AMD发生在病变血管长入黄斑时,导致液体渗漏和出血,损害视力。目前治疗方法是将VEGF抑制剂(例如Lucentis或Avastin)注射到眼睛中,从而减少血管的生长。虽然这种治疗可以稳定甚至改善视觉功能,但50%的治疗眼睛最终会在黄斑中形成纤维化(疤痕)。与愈合伤口的皮肤疤痕不同,黄斑疤痕反而会降低VEGF抑制剂的功效。从病变血管中出现的纤维血管膜最终会破坏整个黄斑。目前,由于对疾病机制的了解不足,没有药物可以预防或治疗这种情况。当称为肌成纤维细胞的细胞浸润并积聚在黄斑中时,湿性AMD中的新血管发展成纤维血管性瘢痕。肌成纤维细胞产生过量的瘢痕形成细胞外基质,如胶原蛋白和纤连蛋白。肌成纤维细胞不存在于健康的视网膜,包括黄斑,我们还不知道它们来自哪里以及它们如何在湿性AMD中被激活。这些知识对于开发湿性AMD和黄斑纤维化的治疗干预至关重要。最近,血管周围间充质干细胞(pMSC),一种独特类型的细胞周围的血管被确定为一个主要来源的肌成纤维细胞损伤诱导的疤痕在多个器官,包括肺,肾,心脏和皮肤。已知这些pMSC可以保护血管并保持其完整性。在损伤期间,它们从血管壁分离并移动到损伤部位,在那里它们参与组织修复和再生。当损伤持续存在或损伤介导的炎症不能迅速消退时,这些pMSC扩增并成为肌成纤维细胞,导致器官纤维化。我们已经发现,在眼睛内,视网膜和脉络膜也含有pMSC的网络。在湿性AMD介导的视网膜纤维化的实验模型中,超过60%的肌成纤维细胞来源于pMSC,并且pMSC的遗传缺失减少了视网膜纤维化。我们的目的是了解为什么pMSCs在湿性AMD中成为肌成纤维细胞,以及我们如何预防或逆转这一过程。我们将使用先进的基因组技术来揭示视网膜纤维化不同阶段病变组织中单个pMSCs的基因表达谱。这将告诉我们哪些pMSC亚型产生肌成纤维细胞以及控制这一过程的途径。我们将在来自视网膜、脉络膜和视网膜瘢痕组织的pMSC培养物中验证这些途径。然后,我们将靶向这些途径与药理学方法,以防止或逆转分化的pMSC成肌纤维细胞使用在体外和体内模型的视网膜纤维化。我们的研究结果将为进一步的研究提供信息,并增强我们对湿性AMD和其他由异常组织修复引起的危及视力的疾病(例如增殖性糖尿病视网膜病变[PDR]和增殖性玻璃体视网膜病变[PVR])中视网膜修复和纤维化的理解。最终,我们的研究将致力于开发有效的视网膜纤维化治疗方法。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Immune Cells in Subretinal Wound Healing and Fibrosis.
Targeting the complement pathway for the management of subretinal fibrosis
靶向补体途径治疗视网膜下纤维化
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    M Chen
  • 通讯作者:
    M Chen
Differential Role of Macrophage and Microglia in Choroidal Neovascularisation-mediated Retinal Fibrosis
巨噬细胞和小胶质细胞在脉络膜新生血管介导的视网膜纤维化中的不同作用
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Szczepan M
  • 通讯作者:
    Szczepan M
Macrophage-to-myofibroblast transition in retinal fibrosis
视网膜纤维化中巨噬细胞向肌成纤维细胞的转变
  • DOI:
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    H Xu
  • 通讯作者:
    H Xu
Old age promotes retinal fibrosis in choroidal neovascularization through circulating fibrocytes and profibrotic macrophages.
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Heping Xu其他文献

Association of TyG index with mortality at 28 days in sepsis patients in intensive care from MIMIC IV database
MIMIC IV 数据库中重症监护脓毒症患者 28 天死亡率与 TyG 指数的相关性
  • DOI:
    10.1038/s41598-025-86746-w
  • 发表时间:
    2025-01-17
  • 期刊:
  • 影响因子:
    3.900
  • 作者:
    Heping Xu;Jinyuan Xie;Yan Xia;Huan Niu;Hong Wang;Feng Zhan
  • 通讯作者:
    Feng Zhan
Associations between triglyceride-glucose body mass index and all-cause mortality in ICU patients with sepsis and acute heart failure
  • DOI:
    10.1186/s12872-025-04804-7
  • 发表时间:
    2025-05-09
  • 期刊:
  • 影响因子:
    2.300
  • 作者:
    Heping Xu;Jinyuan Xie;Huan Niu;Xiongwei Cai;Ping He
  • 通讯作者:
    Ping He
Inflammation in Age-Related Macular Degeneration – Implications for Therapy
年龄相关性黄斑变性的炎症——对治疗的影响
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Mei Chen;Heping Xu
  • 通讯作者:
    Heping Xu
Identification of a new isoform of the murine Sh2d1a gene and its functional implications
鼠 Sh2d1a 基因新亚型的鉴定及其功能意义
  • DOI:
    10.1007/s11427-013-4584-z
  • 发表时间:
    2013
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Longyan Wu;Pei;Weiwei Ma;Coco Chu;Heping Xu;Hai Qi
  • 通讯作者:
    Hai Qi
Topical treatment for AMD: Non-invasive delivery and efficacy of ranibizumab and bevacizumab in rabbit and porcine eyes
AMD 的局部治疗:雷珠单抗和贝伐单抗在兔和猪眼中的无创给药及其疗效
  • DOI:
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    F. Cogan;A. Lynch;Matthew R. Berwick;Anna F. A. Peacock;S. Elsherbiny;Heping Xu;Mei Chen
  • 通讯作者:
    Mei Chen

Heping Xu的其他文献

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