ENDOGENOUS GLUCOSE PRODUCTION DURING AN INTRAVENOUS GLUCOSE TOLERANCE TEST

静脉葡萄糖耐量测试期间内源性葡萄糖的产生

• 批准号: 6118603
• 负责人: P VICINI
• 金额: $ 0.4万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118603
• 关键词: biomedical resource; carbohydrates; diabetes mellitus; endocrine gland /system; female; hormones; human subject; male; model design /development; spectrometry; technology /technique

Recently, a new method based on a two compartment minimal model and deconvolution (Am. J. Physiol. 264: E829-E841, 1993; Comp. Meth. Prog. Biomed. 52: 147-156, 1997) has been proposed to estimate endogenous glucose production (EGP) from labeled IVGTT data. Our aim here is to compare this EGP profile with that independently obtained with the reference method, based on the tracer to tracee ratio (TTR) clamp. An insulin modified (0.03 U/kg body weight infused over 5 minutes) [6,6-2H2]glucose labeled IVGTT (0.33 g/kg of glucose) was performed in ten normal subjects. A second tracer ([U-13C]glucose) was also infused during the test in a variable fashion, so as to clamp endogenous glucose TTR. The TTR clamp was quite successful. As a result, the EGP profile reconstructed from [U-13C]glucose data with Steele's and with Radziuk's models were almost superimposable, thus confirming the substantial model-independence of the method. The deconvolution-obtained EGP profile, c alculated from [6,6-2H2]glucose data, showed remarkable agreement with that obtained from the TTR clamp. Some differences between the two profiles were noted in the estimated basal EGP and in the initial modalities of EGP inhibition. A high inter-individual variability was also observed with both methods in the resumption of EGP to baseline; variability was high both in the timing and the extent of resumption. In conclusion, the use of the two compartment minimal model of the IVGTT and deconvolution allows the estimation of a profile of EGP which is in very good agreement with that independently obtained with a TTR clamp.

最近，一种基于两室最小模型的新方法 和反褶积(Am.J.Physiol.264：E829-E841,1993； 冰毒。程序。生物医学。52：147-156,1997)已提议 根据标记的IVGTT数据估计内源性葡萄糖产生(EGP)。 我们在这里的目标是独立地比较这个EGP配置文件和那个配置文件 用参照法获得的，基于示踪剂的跟踪 比率(Ttr)夹具。改良胰岛素(0.03U/kg体重输注) 超过5分钟)[6，6-2H2]葡萄糖标记的IVGTT(0.33克/公斤葡萄糖) 对10名正常受试者进行了检查。第二种示踪剂 ([U-13C]葡萄糖)在测试期间也被注入变量中 时尚，从而钳制内源性葡萄糖TTR.Ttr夹子是 相当成功。因此，重新构建的EGP配置文件 [U-13C]Steele模型和Radziuk模型的血糖数据是 几乎是重叠的，从而证实了实质性的 方法的模型无关性。反褶积得到的EGP 根据[6，6-2H2]葡萄糖数据计算的图谱显示， 与从TTR钳中获得的结果一致。一些不同点 在估计的基本EGP和 EGP抑制的最初模式。高度的个人化 两种方法的可变性也在恢复时观察到 EGP到基线；时间和时间的变异性都很高 收回的范围。总而言之，两个隔间的使用 IVGTT和反褶积的最小模型允许估计 一份与此非常吻合的特惠计划简介 使用TTR夹具独立获得。