DIABETES & SCIATIC NERVE HYPEREMIA INDUCED BY SURGICAL TRAUMA: DIAB NEUROPATHY

糖尿病

• 批准号: 6118529
• 负责人: Y A IDO
• 金额: $ 0.08万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118529
• 关键词: Mammalia; biological products; biomedical resource; carbohydrates; diabetes mellitus; drug /agent; endocrine gland /system; nervous system; spectrometry

The most widely used methods to assess nerve blood flow in diabetic rats are hydrogen clearance polarography and laser Doppler flowometry, techniques requiring surgical exposure of the nerve. In these experiments, we examined the hypothesis that the trauma of surgical exposure introduces an important and hitherto largely unrecognized variable that could account for discordant reports on nerve blood flow changes induced by diabetes. We used the noninvasive (for sciatic nerve) reference sample micro-sphere method to quantify sciatic nerve blood flow in unexposed vs. surgically exposed nerves in rats with streptozotocin-induced diabetes (at different temperatures and after curarization) and in unexposed vs. surgically exposed nerves in galactose-fed rats. Baseline resting blood flow in unexposed nerves in both animal models of diabetes was either normal or increased (but was decreased in diabetic rats given d-turbocurarine). Furthermore, the normal brisk hyperemic nerve blo od flow response to the minimal trauma associated with surgical exposure of the nerve was markedly impaired in diabetic and in galactose-fed rats. Normalization of the blood flow response to trauma in galactose-fed rats by an aldose reductase inhibitor suggests that the impairment is linked to increased polyol pathway metabolism. These findings 1) confirm our previous findings that sciatic nerve blood flow in diabetic rats is increased or unchanged in unexposed nerves, while also confirming reports that in surgically exposed nerves blood flow is higher in control than in diabetic rats, and 2) indicate that blood flows in surgically exposed nerves are largely a measure of vascular responses to injury rather than (patho)physiological blood flow in undisturbed nerves. Tissue galactitol levels were measured by GC/MS and were elevated in galactose-fed animals but were normalized by aldose reductase inhibitors.

最广泛使用的评估神经血流的方法 糖尿病大鼠的氢清除极谱和激光多普勒 流量计量学，需要外科手术暴露神经的技术。在……里面 在这些实验中，我们检验了一种假设，即 手术暴露带来了一个重要的和迄今为止很大的 无法识别的变量，可能会解释不一致的报告 糖尿病引起的神经血流量改变。我们使用了非侵入性的 (针对坐骨神经)参考样品微球法进行定量 未暴露神经与手术暴露神经中的坐骨神经血流 链脲佐菌素诱导的糖尿病大鼠(不同温度 以及在未暴露与手术暴露的神经中 在喂食半乳糖的大鼠身上。未暴露的基线静息血流量 两种糖尿病动物模型的神经都是正常的或 增加(但在给予d-Turocurarine的糖尿病大鼠中减少)。 此外，正常的充血神经血流反应 与手术暴露神经相关的最小创伤是 在糖尿病大鼠和喂食半乳糖的大鼠中明显受损。 半乳糖喂养创伤后血流反应的正常化 使用醛糖还原酶抑制剂的大鼠表明，这种损害是 与多元醇途径代谢增加有关。这些调查结果1) 证实了我们之前的发现坐骨神经血流 糖尿病大鼠在未暴露的神经中增加或不变，而 也证实了在手术中暴露的神经的血液流动 对照组比糖尿病大鼠更高，2)表明血液 手术暴露的神经中的流量在很大程度上是血管的衡量标准。 对损伤的反应而不是(病理)生理血流 不受干扰的神经。用GC/MS法测定组织中半乳糖醇的含量 在喂食半乳糖的动物中升高，但在 醛糖还原酶抑制剂。