VASCULAR CELLULAR CONSEQUENCES OF HYPERGLYCEMIA

高血糖对血管细胞的影响

• 批准号: 6486712
• 负责人: Y A IDO
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486712
• 关键词: biomedical resource; endocrine gland /system; immunology; inflammation; spectrometry

C-peptide, a cleavage product from the processing of proinsulin to insulin, has been considered to possess little if any biological activity other than its participation in insulin synthesis. Injectin of human C-peptide prevented or attenuated vascular and neural (electrophysiological) dysfunction and impaired Na+- and K+-dependent adenosine triphosphate activity in tissues of diabetic rats. Nonpolar amino acids in the midportion of the peptide were required for these biological effects. Synthetic reverse sequence (retro) and all-D-amino acid (enantio) C-peptides were equipotent to native C-peptide, which indicates that the effects of C-peptide on diabetic vascular and neural dysfunction were mediated by nonchiral interactions instead of sterospecific receptors or binding sites. Tissue sorbitol levels were measured by GC/MS and were not affected by C-peptide.

C-肽，是胰岛素原加工到 胰岛素被认为具有很少的生物活性， 除了参与胰岛素合成外，还具有其他活性。 注射液 人C肽预防或减弱血管和神经 （电生理）功能障碍和受损的Na+和K+依赖性 糖尿病大鼠组织中腺苷三磷酸活性。 非极性 肽的中间部分的氨基酸是这些所需的。 生物效应。 合成反向序列（逆向）和 全-D-氨基酸（对映体）C-肽与天然C-肽等效。 提示C肽对糖尿病患者的作用 血管和神经功能障碍是由非手性 相互作用而不是立体特异性受体或结合位点。 通过GC/MS测量组织山梨醇水平，并且不受 C肽