PREVENTION OF VASCULAR & NEURAL DYSFUNCTION IN DIABETIC RATS BY C-PEPTIDE

预防血管疾病

• 批准号: 6118528
• 负责人: Y A IDO
• 金额: $ 0.08万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118528
• 关键词: Mammalia; biological products; biomedical resource; carbohydrates; cardiovascular system; diabetes mellitus; drug /agent; endocrine gland /system; hormones; proteins; spectrometry

C-peptide, a cleavage product from the processing of proinsulin to insulin, has been considered to possess little if any biological activity other than its participation in insulin synthesis. Injection of human C-peptide prevented or attenuated vascular and neural (electro-physiological) dysfunction and impaired Na+- and K+-dependent adenosine triphosphate activity in tissues of diabetic rats. Non-polar amino acids in the mid-portion of the peptide were required for these biological effects. Synthetic reverse sequence (retro) and all-D-amino acid (enantio) C-peptides were equipotent to native C-peptide, which indicates that the effects of C-peptide on diabetic vascular and neural dysfunction were mediated by non-chiral interactions instead of stero-specific receptors or binding sites. Tissue sorbitol levels were measured by GC/MS and were not affected by C-peptide.

C-肽，是胰岛素原加工到 胰岛素被认为具有很少的生物活性， 除了参与胰岛素合成外，还具有其他活性。 注射 人C肽预防或减弱血管和神经 （电生理）功能障碍和受损的Na+和K+依赖性 糖尿病大鼠组织中腺苷三磷酸活性。 在肽的中间部分需要非极性氨基酸 这些生物效应。 合成反向序列（逆向）和 全-D-氨基酸（对映体）C-肽与天然C-肽等效。 提示C肽对糖尿病患者的作用 血管和神经功能障碍是由非手性 相互作用，而不是立体特异性受体或结合位点。 通过GC/MS测量组织山梨醇水平，并且不受 C肽