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HIGH FREQUENCY EPR OF TYROSYL RADICAL OF RIBONUCLEOTIDE REDUCTASE

HIGH FREQUENCY EPR OF TYROSYL RADICAL OF RIBONUCLEOTIDE REDUCTASE
核糖核苷酸还原酶酪氨酰自由基的高频 EPR
批准号：
6279698
负责人：
GARY J. GERFEN
金额：
$0.59万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-05-01 至 1999-04-30
来源：
https://reporter.nih.gov/project-details/6279698
关键词：
biological products biomedical equipment development biomedical resource enzymes technology /technique

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Ribonucleotide reductase (RNR) from Escherichia coli catalyzes the conversion of nucleotides to deoxynucleotides and is composed of two homodimeric subunits: R1 and R2. We have performed several experiments with this protein over the past three years. Initially, we obtained a 140 GHz spectrum which revealed a well resolved g-anisotropy and hyperfine couplings to the 3,5-aromatic protons as well as the P-CH2-The simulated 140 GHz lineshape is in excellent agreement with the experimental spectrum and a comparison of the 9 and 140 GHz spectra shows the advantage of recording this line shape at high field.
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