HIGH FREQUENCY EPR STUDIES OF VOLTAGE DEPENDENT ION CHANNELS
HIGH FREQUENCY EPR STUDIES OF VOLTAGE DEPENDENT ION CHANNELS
批准号:
6281741
负责人:
GARY J. GERFEN
金额:
$0.19万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-05-05 至 2000-04-30
中文摘要
粘菌素Ia是一种含有626个氨基酸的细菌蛋白质,可以杀死细胞
英文摘要
Colicin Ia is a 626 amino acid bacterial protein which kills cells
by disrupting active transport and by inducing the leakage of ions.
It has been shown that this activity derives from the ability of
Colicin Ia to form voltage-dependent, relatively non-selective
ion-permeable channels in the bacterial plasma membrane. The efficacy
of Colicin Ia is remarkable, in that a single protein molecule in the
membrane is enough to kill the cell. In addition, the voltage
dependent channel forming behavior can be reproduced in artificial
phospholipid bilayer membranes and vesicles, thus providing an ideal
system for the general study of ion channel physics. High frequencey
EPR studies of spin-labeled colicin Ia molecules imbedded in vesicles
is an ideal means to address structural details of voltage-dependent
ion channel structure. An overview of the proposed experimental
protocol is as follows. A paramagnetic nitroxide spin label is
introduced into the colicin protein at a residue position of interest.
The colicin is then imbedded in membrane vesicles followed by rapid
mixing with the depsipeptide valinomycin to establish a transmembrane
potential. At this point the system can be rapidly frozen to lock the
protein conformational state in place followed by low temperature EPR
spectroscopy. Alternatively, solution phase EPR spectra may be
obtained. In either case, the EPR spectrum of the nitroxide spin
label is influenced by the polarity of the environment, and thus
provides a means to establish if the label is outside or within (and
perhaps how deep within) the vesicle bilayer. This in turn leads to
information concerning the voltage-dependent structure of the protein
and how it forms the ion channel.
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Phosphoregulation of the Kinesin Motor Domain: Structure, Dynamics and Function
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批准号:8238617
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项目类别:
-
资助金额:$46.78万
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财政年份:2012
-
负责人:GARY J. GERFEN
-
依托单位:
Phosphoregulation of the Kinesin Motor Domain: Structure, Dynamics and Function
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批准号:8658438
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项目类别:
-
资助金额:$44.42万
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财政年份:2012
-
负责人:GARY J. GERFEN
-
依托单位:
Phosphoregulation of the Kinesin Motor Domain: Structure, Dynamics and Function
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批准号:8462999
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项目类别:
-
资助金额:$42.87万
-
财政年份:2012
-
负责人:GARY J. GERFEN
-
依托单位:
Proton Crystallography of Membrane Proteins Using High Frequency ENDOR
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批准号:7279333
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项目类别:
-
资助金额:$17.87万
-
财政年份:2006
-
负责人:GARY J. GERFEN
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依托单位:
Proton Crystallography of Membrane Proteins Using High Frequency ENDOR
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批准号:7011282
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项目类别:
-
资助金额:$18.4万
-
财政年份:2006
-
负责人:GARY J. GERFEN
-
依托单位:
Proton Crystallography of Membrane Proteins Using High Frequency ENDOR
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批准号:7492999
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项目类别:
-
资助金额:$17.53万
-
财政年份:2006
-
负责人:GARY J. GERFEN
-
依托单位:
Proton Crystallography of Membrane Proteins Using High Frequency ENDOR
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批准号:7681485
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项目类别:
-
资助金额:$17.53万
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财政年份:2006
-
负责人:GARY J. GERFEN
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依托单位:
ADVANCED EPR STUDIES OF B12 DEPENDENT ENZYMES
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批准号:6039831
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项目类别:
-
资助金额:$19.24万
-
财政年份:2000
-
负责人:GARY J. GERFEN
-
依托单位:
ADVANCED EPR STUDIES OF B12 DEPENDENT ENZYMES
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批准号:6628902
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项目类别:
-
资助金额:$20.73万
-
财政年份:2000
-
负责人:GARY J. GERFEN
-
依托单位:
ADVANCED EPR STUDIES OF B12 DEPENDENT ENZYMES
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批准号:6498827
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项目类别:
-
资助金额:$20.16万
-
财政年份:2000
-
负责人:GARY J. GERFEN
-
依托单位:
ADVANCED EPR STUDIES OF B12 DEPENDENT ENZYMES
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批准号:6701365
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项目类别:
-
资助金额:$21.34万
-
财政年份:2000
-
负责人:GARY J. GERFEN
-
依托单位:
ADVANCED EPR STUDIES OF B12 DEPENDENT ENZYMES
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批准号:6351343
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项目类别:
-
资助金额:$19.62万
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财政年份:2000
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负责人:GARY J. GERFEN
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依托单位:
EPR: ADENOSYLCOBALAMIN DEPENDENT ENZYME RIBONUCLEOTIDE TRIPHOSPHATE REDUCTASE
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批准号:6281740
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项目类别:
-
资助金额:$0.19万
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财政年份:1998
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负责人:GARY J. GERFEN
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依托单位:
ID 4 GLUTAMYL RADICAL AS INTERMEDIATE IN CARBON SKELETON REARRANGEMENT
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批准号:6281739
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项目类别:
-
资助金额:$0.19万
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财政年份:1998
-
负责人:GARY J. GERFEN
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依托单位:
AZIDO INHIBITOR OF RIBONUCLEOTIDE REDUCTASE
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批准号:6279699
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项目类别:
-
资助金额:$0.59万
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财政年份:1998
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负责人:GARY J. GERFEN
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依托单位:
WORKSHOP ON HIGH FREQUENCY EPR SPECTROSCOPY
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批准号:6121176
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项目类别:
-
资助金额:$1.56万
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财政年份:1998
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负责人:GARY J. GERFEN
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依托单位:
PURCHASE & INSTALL HIGH FIELD MAGNET FOR CONSTRUCTION OF 140 GHZ EPR SPECT
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批准号:6121149
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项目类别:
-
资助金额:$0.39万
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财政年份:1998
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负责人:GARY J. GERFEN
-
依托单位:
FLUOROMETHYLENE DEOXYCYTIDINE DIPHOSPHATE(FMCDP) INHIBIT RIBONUCLEOTIDE REDUCTAS
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批准号:6279700
-
项目类别:
-
资助金额:$0.59万
-
财政年份:1998
-
负责人:GARY J. GERFEN
-
依托单位:
PURCHASE & INSTALL HIGH FIELD MAGNET FOR CONSTRUCTION OF 140 GHZ EPR
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批准号:6281725
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项目类别:
-
资助金额:$1.25万
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财政年份:1998
-
负责人:GARY J. GERFEN
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依托单位:
HIGH FREQUENCY EPR OF TYROSYL RADICAL OF RIBONUCLEOTIDE REDUCTASE
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批准号:6279698
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项目类别:
-
资助金额:$0.59万
-
财政年份:1998
-
负责人:GARY J. GERFEN
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依托单位:
海外基金