SELF ASSOCIATION OF COPPER(II) TETRAPHENYLPORPHINE IN LIPID BILAYERMEMBRANES

四苯基卟啉铜(II)在脂质双层膜中的自缔合

• 批准号: 6279876
• 负责人: WITOLD K SUBCZYNSKI
• 金额: $ 0.11万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279876
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; lipids

Copper (II) tetraphenylporphine (CuTPP) has been found to partition favorably into dimyristoylphosphatidylcholine (DMPC) vesicles. EPR spectra of oriented DMPC membranes together with computer simulations show that the CuTPP is preferentially oriented with the plane of the molecule perpendicular to the bilayer surface at low (less than or equal to 1/100) CuTPP/DMPC molar ratio. However, for a high (ca. 1/25) CuTPP/DMPC molar ratio, the plane of the molecule becomes parallel to the membrane surface. Measurements of the collision rate between CuTPP and the nitroxide moiety of stearic acid spin labels located at different depths in the membrane using saturation recovery EPR spectroscopy led to the conclusion that the change of the CuTPP orientation is the result of self-association (crystallization) of CuTPP molecules near the lipid bilayer center. The self-association process depends both on the CuTPP/DMPC molar ratio and the time after sample preparation.

铜(II)四苯基卟啉(CuTPP)已被发现 二肉豆蔻基磷脂酰胆碱(DMPC)的有利分配 水泡。定向DMPC膜的EPR谱 计算机模拟表明，CuTPP是优先定向的 分子的平面垂直于双层表面 低(小于或等于1/100)CuTPP/DMPC摩尔比。然而， 对于较高的CuTPP/DMPC摩尔比(约1/25)， 分子变得与膜表面平行。测量 硬脂酸中含氮部分与CuTPP的碰撞速率 位于膜中不同深度的酸自旋标记使用 饱和恢复电子顺磁共振波谱的结论是 CuTPP取向的改变是自缔合的结果 (结晶)CuTPP分子在脂质双层中心附近。 自缔合过程取决于CuTPP/DMPC摩尔比 比例和样品制备后的时间。