SPIN LABEL EFFECTS OF MEMBRANE SPANNING PEPTIDE L24 & LA12 ON POPC MEMBRANES

在此之前，我们研究了膜跨越的影响。 带有KKL24KK序列的L24和带有序列LA12的L24 KK(LA)12KK在POPC膜上。我们使用了含氮氧化合物的PC自旋标记 部分位于5-、7-、10-、12-和16位 使脂双层有序，烷基链运动自由度降低， 显著提高了膜的疏水性。 内饰。今年，我们通过研究 铁氰化钾离子溶于水后进入POPC-多肽的可及性 膜。在多肽存在的情况下，药物的渗透水平 铁氰化物显著降低。这些结果是在 在-150℃下得到的液相膜与先前获得的膜一致 疏水性特征。我们的研究让我们对蛋白质-蛋白质有了深入的了解 膜上的相互作用和膜蛋白在膜上的折叠 膜，因为它们与多肽结构，表面粗糙度， 和两个肽排泄物或一个肽的结构构象 和脂类，当它们相邻地放置在膜中时。

Previously we investigated the effects of membrane-spanning peptides L24 with a sequence KKL24KK and LA12 with a sequence KK(LA)12KK on POPC membranes. We used PC-spin labels with nitroxide moiety located at the 5-, 7-, 10-, 12-, and 16-positions Both peptides ordered the lipid bilayer, decreased motional freedom of alkyl chains, and significantly increased the hydrophobicity of the membrane interior. This year we confirmed our previous results by studying the accessibility of ferricyanide ion dissolved in water into POPC-peptide membranes. In the presence of peptide the level of penetration of ferricyanide decreased significantly. These results obtained in fluid-phase membranes are consistent with previously obtained at -150C hydrophobicity profiles. Our studies give insight to protein-protein interaction in the membrane, and folding of membrane proteins in the membrane as they relate to the peptide structure, surface roughness, and structural conformability of two peptide drains or of a peptide and lipid when they are adjacently placed in the membrane.