CHARACT OF DIFFRACTION FROM CRYSTALS OF 70S RIBOSOMES FROM T THERMOPHILUS

嗜热杆菌 70S 核糖体晶体的衍射特征

• 批准号: 6119574
• 负责人: Charles J Wilson
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119574
• 关键词: biomaterials; genome

We aim to determine the structure of the 70S ribosome from Thermus thermophilus complexed with mRNA and tRNA analogs. Recently we have obtained improved crystals that diffract to at least 8 E resolution. Experiments will focus on (1) improving the diffraction quality and cryoprotection of these new crystals, (2) collection of a new native data set, and (3) extending the molecular replacement phases to the diffraction limit of the crystals. We will pursue MAD and MIRAS phasing by means of heavy atom clusters and conventional heavy-atom compounds to derivatize crystals.

我们的目的是确定来自热菌的70S核糖体的结构