CORE C: IMAGING CORE

核心 C：成像核心

• 批准号: 7715333
• 负责人: Charles J Wilson
• 金额: $ 12.15万
• 依托单位: UNIVERSITY OF TEXAS SAN ANTONIO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715333
• 关键词: Achievement; Address; Area; Awareness; Biological Sciences; Biology; Budgets; Class; Collaborations; Communities; Computer Retrieval of Information on Scientific Projects Database; Computer software; Confocal Microscopy; Data; Educational Status; Educational workshop; Equilibrium; Facility Construction Funding Category; Fire - disasters; Four-dimensional; Funding; Grant; Human Resources; Image; Image Analysis; In Situ; Insecta; Institution; Joints; Journals; Laboratories; Lasers; Maintenance; Manuscripts; Modeling; Neurons; Numbers; Photons; Process; Publications; Radiation; Reporting; Research; Research Personnel; Resources; Safety; Sister; Source; System; Technology; Testing; Texas; Time; Tissues; Training; United States National Institutes of Health; Universities; Work; experience; falls; instrument; instrumentation; interest; member; research study; technology development; tool; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There has been no change in the support of the key personnel since the last reporting period. There will not be any significant change in the level of effort for key personnel in the next budget period. The estimated unobligated balance will not be greater than 25% of the current year's budget. A. Specific Aims: The aims of this project have not changed. We have finished the construction of the first of our 2 photon systems and training of users is underway. B. Progress this year: With the first of our systems completed, the construction of the second system will be finished over the course of the summer. There have been, as to be expected, a number of kinks in the system that have needed to be worked out. We continue to deal with these as they arise. We expect that this year we will finish addressing any issues with the systems and be in full swing by Fall. Training on the system has been intermittent while we have dealt with technical issues. The labs that are currently undergoing training include the Palladini, Wilson, Santa Maria, Jaffe, and Sutter labs. We have complied fully with all safety requirements, including the completion of the UTSA radiation safety course and test. We are in the process of taking the final steps requested by our campus laser safety office in order to be in complete compliance with our campus safety requirements for rooms containing class 4 lasers (as the 2P laser is when uncovered for routine maintenance and alignment). These final steps include the purchase of fire proof curtains for our doors and the installation of 'laser in use' signs to be active during times of laser maintenance. In addition, the imaging facility has undergone our first yearly inspection for lab safety. No additional steps were required on our part from this assessment and we are in full compliance. The BioRad 1024 has continued to be in heavy use this last year. It has provided key data for a number of studies that were presented at a variety of scientific meetings. The primary users of this system have been members of Dr. Brenda Claiborne and Dr.Martha Lundell. We expect that this system will eventually be supplanted by the new Zeiss 510 Meta system that the RCMI imaging core has acquired from the biology department as maintenance for the BioRad will not be available after the year 2008. In addition, the technological advantages of the new Zeiss make this system more desirable from a research standpoint. Since the installation of the new Zeiss 510 Meta confocal, this system has been the workhorse for training in imaging principles and has proven invaluable. Prior to its install few labs on campus had any previous experience with imaging in general, and in particular, had not as yet included confocal microscopy as part of their research tool set. The Zeiss has dramatically changed this on campus. There are now currently over 12 labs which have received complete training, not only on the level of 'pushing buttons' on the Zeiss, but through Dr. Witt have received detailed training in principles of imaging. This level of training has allowed labs to become fully self-sufficient with the instrumentation and the first publications from the use of the Zeiss are now being prepared and submitted. Training on the Zeiss is also serving as an excellent introduction to imaging for the user prior to beginning work on the two photon systems. This year has also seen the submission of the first manuscripts to come from our nascent core. These include Dr. Witt's manuscript to The Journal of Experimental Biology in collaboration with Dr. Kerry Landman of The University of Melbourne and a second manuscript in submission to the Journal of Experimental and Applied Arachnology. This latter is the culmination of a collaboration between Drs Witt and Dr. Andrew Li of the USDA Insect Laboratory of Kerville, Texas. C. Significance: The advent of 2 photon imaging has revolutionized research in the Life Sciences enabling us for the first time to perform experiments in the native tissue and in real time. The significance of this technological achievement cannot be overstated and the move toward 3 and 4 dimensional imaging in situ will only gain momentum as this technology becomes more commonly available. It is more essential than ever that UTSA pursues the development of this technology to its fullest. D. Plans for the Upcoming Year: Our first priorities will be to complete training for our first round of users on the finished 2P system and to complete the construction of our second 2P system. Training will be provided by Dr. Witt and will include safety awareness in addition to the use of the instrument. The completion of the second system will be a joint effort between Drs. Wilson and Witt and the time frame for completion will be the Fall 2008. As mentioned, the final steps for bring our imaging facility into compliance with our campus Laser Safety Office will also be completed this summer. We have already hosted our first guest speaker, Dr. Tomasz Zal, of MD Anderson. His talk not only drew a full capacity audience, but he spent the afternoon with us in the imaging center and provided some excellent tips and expertise. We plan to host additional guest speakers, such as Dr. Zal, who will speak on the topic of 2 photon imaging in key interest areas of research. We will host one workshop on imaging that will be opened first to UTSA community and then outside attendees if space permits. This year will also see a strengthened alliance with our sister core The Biocomputational Initiative as we will also host our second workshop on quantitative imaging analysis in conjunction with Bitplane Inc, developers of the leading 3D/4D imaging analysis software, Imaris. Dr. Witt is also coordinating a formal collaboration between the RCMI Imaging core, Dr. James Bower of the Biocomputational Initiative, and Bitplane, Inc to partner Imaris analysis software with Genesis neuronal modeling software.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 自上一报告期以来，关键人员的支持情况未发生变化。 下一个预算期间关键人员的工作水平不会有任何重大变化。 预计未承付余额将不超过当年预算的25%。 A. 具体目标：该项目的目标没有改变。我们已经完成了第一个 2 个光子系统的构建，用户培训正在进行中。 B. 今年的进展：随着第一个系统的完成，第二个系统的建设将在夏季完成。正如预期的那样，系统中存在许多需要解决的问题。当这些问题出现时，我们将继续进行处理。我们预计今年我们将完成系统问题的解决，并在秋季全面展开。当我们处理技术问题时，系统培训一直是断断续续的。目前正在接受培训的实验室包括 Palladini、Wilson、Santa Maria、Jaffe 和 Sutter 实验室。我们已完全遵守所有安全要求，包括完成 UTSA 辐射安全课程和测试。我们正在采取校园激光安全办公室要求的最后步骤，以便完全符合校园对包含 4 级激光器的房间的安全要求（因为 2P 激光器在日常维护和校准时未覆盖）。这些最后步骤包括为我们的门购买防火窗帘以及安装“激光使用中”标志，以便在激光维护期间激活。此外，成像设施还接受了我们第一次实验室安全年度检查。此次评估中我们不需要采取任何额外措施，并且我们完全遵守规定。 BioRad 1024 去年继续得到大量使用。它为在各种科学会议上发表的多项研究提供了关键数据。该系统的主要用户是 Brenda Claiborne 博士和 Martha Lundell 博士的成员。我们预计该系统最终将被 RCMI 成像核心从生物部门获得的新型 Zeiss 510 Meta 系统取代，因为 BioRad 在 2008 年之后将无法进行维护。此外，从研究的角度来看，新型 Zeiss 的技术优势使该系统更受欢迎。 自从安装新型蔡司 510 Meta 共焦以来，该系统一直是成像原理培训的主力，并被证明具有无价的价值。在安装之前，校园内很少有实验室具有任何一般成像经验，特别是尚未将共焦显微镜作为其研究工具集的一部分。蔡司极大地改变了校园的这一状况。目前有超过 12 个实验室接受了完整的培训，不仅是蔡司“按钮”级别的培训，而且还通过 Witt 博士接受了成像原理方面的详细培训。 这种水平的培训使实验室能够完全自给自足地使用仪器，并且目前正在准备和提交使用蔡司的第一批出版物。在开始使用两个光子系统之前，对蔡司的培训也可以作为用户对成像的一个很好的介绍。 今年我们还提交了来自我们新生核心的第一份手稿。 其中包括 Witt 博士与墨尔本大学的 Kerry Landman 博士合作发表在《实验生物学杂志》上的手稿，以及提交给《实验与应用蜘蛛学杂志》上的第二份手稿。后者是德克萨斯州克维尔美国农业部昆虫实验室的 Witt 博士和 Andrew Li 博士合作的成果。 C. 意义：2 光子成像的出现彻底改变了生命科学研究，使我们第一次能够在天然组织中实时进行实验。这项技术成就的重要性怎么强调都不为过，随着这项技术变得更加普遍，向 3 维和 4 维原位成像的发展只会获得动力。 UTSA 比以往任何时候都更需要充分发展这项技术。 D. 来年计划：我们的首要任务是完成对已完成的 2P 系统的第一轮用户的培训，并完成第二个 2P 系统的构建。培训将由 Witt 博士提供，除了仪器使用之外，还将包括安全意识。第二个系统的完成将是博士们的共同努力。 Wilson 和 Witt 的完成时间为 2008 年秋季。如前所述，使我们的成像设施符合校园激光安全办公室要求的最后步骤也将于今年夏天完成。我们已经接待了我们的第一位演讲嘉宾，来自 MD 安德森的 Tomasz Zal 博士。他的演讲不仅吸引了满座的听众，而且他在成像中心与我们一起度过了整个下午，并提供了一些出色的技巧和专业知识。我们计划邀请更多的演讲嘉宾，例如 Zal 博士，他将就关键研究领域的 2 光子成像主题发表演讲。我们将举办一场成像研讨会，该研讨会将首先向 UTSA 社区开放，然后在空间允许的情况下向外部与会者开放。今年我们还将与我们的姊妹核心生物计算计划加强联盟，因为我们还将与领先的 3D/4D 成像分析软件 Imaris 的开发商 Bitplane Inc 联合举办第二届定量成像分析研讨会。 Witt 博士还负责协调 RCMI 成像核心、生物计算计划的 James Bower 博士和 Bitplane, Inc 之间的正式合作，以将 Imaris 分析软件与 Genesis 神经元建模软件合作。