STRUCTURE DETERMINATION OF CATALYTIC, METAL BINDING & ANTI BACTERIAL ANTIBODIES

我们使用的是43 C9抗体，它是针对一种 催化酰胺和酯水解的膦酰胺半抗原 债券，作为支架的金属网站的设计与最终的 设计具有催化活性金属的位点的目标。 晶体结构之间侧链位置的差异 天然43 C9和Zn结合突变体的结构已被用于 确定锌结合位点的残基。 另外我们有 能够模拟半抗原结合的基础上， 构建了43 C9-对硝基苯酚二元配合物，并用于鉴定 其他突变将改善金属结合位点。 在 未来，我们希望进一步探索这个锌结合位点， 阐明其他潜在的催化金属结合的结构 网站.

We are using the 43C9 antibody, which was raised against a phosphonamidate hapten to catalyze the hydrolysis of amide and ester bonds, as scaffold for the design of metal sites with the ultimate goal of designing a site with a catalytically active metal. Differences in side chain positions between the crystallographic structures of native 43C9 and a Zn-binding mutant have been used to identify residues that will the Zn-binding site. In addition, we have been able to model the hapten binding based on the crystallographic structure 43C9-p-nitrophenol binary complex, and used it to identify other mutations that will improve the metal binding site. In the future we hope to explore this Zn-binding site further and to elucidate the structures of other potential catalytic metal binding sites.