DATA COLL ON CO CRYSTAL BETWEEN ACTIVE RAS & RAS INTERACTING DOMAIN OF RALGDS

RAS蛋白是一种鸟苷三磷酸酶，是一种分子 切换信号转导通路，控制细胞生长和 差异化。在人类细胞中，关键区域的点突变 RAS可导致细胞生长失控，或肿瘤。我们正在努力 解开活性RAS蛋白之间的复合体结构 (RAS-GMPPNP)和RalGDS的RAS相互作用结构域(RalGDS-RID)，a Ral蛋白(RAS样蛋白)的GDP解离刺激物。我们的 Lab已经解决了有源RAS结构和结构 RalGDS-RID使用MAD方法。我们想要获得一种共晶 数据集，这将允许我们通过以下方式解决复杂的结构 分子替换法。

Ras protein, a guanosine triphosphatase (GTPase), is a molecular switch in signal transduction pathways that control cell growth and differentiation. In human cells, point mutations in crucial regions of Ras can cause uncontrolled cell growth, or tumors. We are trying to solve the structure of the complex between active Ras protein (Ras-GMPPNP) and the Ras interacting domain of RalGDS (RalGDS-RID), a GDP dissociation stimulator of Ral protein (Ras like protein). Our lab has solved the active Ras structure and the structure of RalGDS-RID with the MAD method. We would like to obtain a co-crystal data set, which will allow us to solve the structure of the complex by the Molecular Replacement method.