CRYOPRESERVED CRYSTALS OF ZN ?2 GLYCOPROTEIN, INVASIN & FC?R

ZN ?2 糖蛋白、Invasin 的冷冻保存晶体

• 批准号: 6119438
• 负责人: Pamela J Bjorkman
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119438
• 关键词: biomaterials; proteins

Data will be measured from crystals of Zn-alpha2-glycoprotein (ZAG), invasin, and the neonatal Fc receptor (FcRn). ZAG is related to class I MHC molecules and has been suggested to play a role in breast cancer. We have solved the structure to 2.9 E using x-rays generated from an in-house rotating anode, and intend to collect high resolution data from cryopreserved crystals in order to interpret electron density that appears to represent a small molecule ligand bound to ZAG. Invasin is 120 kDa protein present on the outer membrane of the enteropathogenic bacterium Yersinia pseudotuberculosis. Invasin binds to several b1 integrins and this interaction mediates the penetration of the bacteria into the host cell. Crystals of an invasin fragment have been grown and a preliminary 2.2 E crystal structure has been solved. FcRn mediates the transport of IgG from the mother to the developing fetus and protects IgG from protein catabolism. We have determined the structure of human FcRn to 2.5 E resolution using data collected at SSRL.

数据将从锌-α2-糖蛋白的晶体中测量 (ZAG)、INVASIN和新生儿Fc受体(FcRN)。ZAG是相关的 到I类MHC分子，并被建议在 乳腺癌。我们已经用X射线解算出了2.9E的结构 由内部旋转的阳极产生，并打算收集高 来自低温保存的晶体的分辨率数据，以便解释 似乎代表小分子配体的电子密度 注定要折腾。血管紧张素是一种存在于外层的120 kDa蛋白质 肠道致病菌耶尔森氏菌的膜 假性肺结核。Invesin与几个b1整合素结合，这 相互作用调节细菌对宿主的渗透 手机。已经生长出一种侵袭素片段的晶体，并且一种 初步确定了2.2E的晶体结构。FcRN介导 免疫球蛋白从母亲到发育中的胎儿的运输和 保护免疫球蛋白免受蛋白质分解代谢。我们已经确定了 利用在以下地点收集的数据将人类FcRN的结构调整为2.5 E分辨率 SSRL.