MEASUREMENT OF METAL PROTEIN COMPLEX USING SMALL ANGLE XRAY SCATTERING

使用小角 X 射线散射测量金属蛋白复合物

• 批准号: 6322189
• 负责人: HARRY B GRAY
• 金额: --
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6322189
• 关键词: biomaterials; proteins; structural biology

We need to measure the radius of gyration of met-myoglobin that has been reacted with an excess of [Co(acacen)(NH3) 2]Cl (acacen meaning acetylacetonate diimine). Our work shows that the resulting protein product is partially unfolded and is bound to 6 cobalt complexes. Possible applications for such species range from molten globules as toxin like pro-drugs to improved protein production in industrial scales. They may be instrumental in the study of protein aggregation in connection with amyloidases or the prion disease, and in the study of protein folding. A radius of gyration --provided by SAXS-- will provide us with the first indication of the underlying structure of this protein-metal complex.

我们需要测量高铁肌红蛋白的回转半径 与过量的[Co（acacen）（NH3）2]Cl（acacen 意思是乙酰丙酮二亚胺）。 我们的工作表明， 蛋白质产物是部分解折叠的，并与6钴结合 配合物 这种物质的可能应用范围从熔融的 作为毒素样前药的小球， 工业规模。 它们可能有助于蛋白质的研究 与淀粉样蛋白酶或朊病毒病有关的聚集，和 蛋白质折叠的研究。 旋转半径，由 SAXS--将为我们提供潜在的 这种蛋白质-金属复合物的结构。