CONTINUING DEVELOPMENT OF MIXER SPRAYER AND FLASH

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 摘要： 在过去的两年中，从我们在开发“宏观”混合器/喷雾器装置方面的进展中获得的知识已经被应用于开发该技术的微流体版本（在下面充分描述）。 宏观混合器/喷雾器仍然可用于测试改进方法的新方法（例如，测试新的网格支持和处理以改善亲水性），因为它对于常规操作更稳健。 此外，这种混合器/喷雾器对于不需要短时间间隔（例如> 200毫秒）的实验是有用的。 目前正在撰写描述这项工作的手稿。 用于执行时间分辨低温电子显微术的第二种技术涉及闪烁包含光反应笼状反应物的网格，因为它们被投入到冷冻剂中。 我们已经完成了这项技术的实施，Tapu Shaanxi博士撰写了这项工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: Knowledge gained from our progress on developing a "macroscopic" mixer/sprayer device over the past two years has been applied to developing a microfluidic version of this technology (described fully below). The macroscopic mixer/sprayer continues to be useful for testing new approaches for improving the methodology (e.g. testing of new grid supports and treatments to improve hydrophilicity) as it is more robust for routine operation. Also, this mixer/sprayer is useful for experiments that do not require short time intervals (e.g > 200 milliseconds). A manuscript describing this work is currently being written. A second technique for performing time resolved cryo-electron microscopy involves flashing grids containing photo-reactive caged reactants as they are being plunged into cryogen. We have finalized our implementation of this technology and Dr. Tapu Shaikh wrote up the work.