CONTINUING DEVELOPMENT OF MIXER SPRAYER AND FLASH

混合喷雾器和闪蒸器的持续开发

• 批准号: 7954574
• 负责人: TERENCE C WAGENKNECHT
• 金额: $ 13.4万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954574
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Development; Devices; Funding; Grant; Imagery; Institution; Knowledge; Manuscripts; Methodology; Microfluidics; Minor; Modification; Operative Surgical Procedures; Publications; Research; Research Personnel; Resources; Source; Techniques; Technology; Testing; Time; United States National Institutes of Health; Work; Writing; abstracting; hydrophilicity; improved; millisecond; novel strategies; research study; time interval

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: Knowledge gained from our progress on developing a "macroscopic" mixer/sprayer device over the past two years has been applied to developing a microfluidic version of this technology (described fully below. The macroscopic mixer/sprayer continues to be useful for testing new approaches for improving the methodology (e.g. testing of new grid supports and treatments to improve hydrophilicity) as it is more robust for routine operation. Also, this mixer/sprayer is useful for experiments that do not require short time intervals (e.g > 200 milliseconds). A manuscript describing this work is currently being written. A second technique for performing time resolved cryo-electron microscopy involves flashing grids containing photo-reactive caged reactants as they are being plunged into cryogen. We have finalized our implementation of this technology and Dr. Tapu Shaikh has submitted a manuscript that has been provisionally accepted for publication pending minor modifications.

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