CONTINUING DEVELOPMENT OF MIXER SPRAYER AND FLASH

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 摘要： 过去两年我们在开发“宏观”混合器/喷雾器装置方面取得的进展所获得的知识已应用于开发该技术的微流体版本（下文将详细描述）。宏观混合器/喷雾器继续可用于测试改进方法的新方法（例如测试新的网格支撑和改善亲水性的处理），因为它对于常规操作更加稳健。此外，该混合器/喷雾器对于不需要短时间间隔的实验非常有用（例如> 200 毫秒）。目前正在撰写描述这项工作的手稿。 执行时间分辨冷冻电子显微镜的第二种技术涉及在将含有光反应笼式反应物的反应物投入冷冻剂时闪烁网格。 我们已经完成了这项技术的实施，Tapu Shaikh 博士已提交了一份手稿，该手稿已暂时接受出版，等待稍作修改。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: Knowledge gained from our progress on developing a "macroscopic" mixer/sprayer device over the past two years has been applied to developing a microfluidic version of this technology (described fully below. The macroscopic mixer/sprayer continues to be useful for testing new approaches for improving the methodology (e.g. testing of new grid supports and treatments to improve hydrophilicity) as it is more robust for routine operation. Also, this mixer/sprayer is useful for experiments that do not require short time intervals (e.g > 200 milliseconds). A manuscript describing this work is currently being written. A second technique for performing time resolved cryo-electron microscopy involves flashing grids containing photo-reactive caged reactants as they are being plunged into cryogen. We have finalized our implementation of this technology and Dr. Tapu Shaikh has submitted a manuscript that has been provisionally accepted for publication pending minor modifications.