CONTINUING DEVELOPMENT OF MIXER SPRAYER AND FLASH

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 摘要： 在过去两年中，我们在开发“宏观”混合器/喷雾器设备方面取得的进展所获得的知识已被应用于开发这项技术的微流控版本(在下文全面描述)。宏观混合器/喷雾器继续用于测试改进方法的新方法(例如，测试新的格栅支撑物和处理以改善亲水性)，因为它对常规操作更坚固。此外，这种混合器/喷雾器对于不需要短时间间隔(例如200毫秒)的实验也很有用。描述这项工作的手稿目前正在撰写中。 进行时间分辨冷冻电子显微镜的第二种技术包括闪光栅格，其中包含光反应笼状反应物，因为它们被插入到制冷剂中。我们已经完成了这项技术的实施，Tapu Shaikh博士已经提交了一份手稿，该手稿已暂时接受出版，等待进行微小的修改。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: Knowledge gained from our progress on developing a "macroscopic" mixer/sprayer device over the past two years has been applied to developing a microfluidic version of this technology (described fully below. The macroscopic mixer/sprayer continues to be useful for testing new approaches for improving the methodology (e.g. testing of new grid supports and treatments to improve hydrophilicity) as it is more robust for routine operation. Also, this mixer/sprayer is useful for experiments that do not require short time intervals (e.g > 200 milliseconds). A manuscript describing this work is currently being written. A second technique for performing time resolved cryo-electron microscopy involves flashing grids containing photo-reactive caged reactants as they are being plunged into cryogen. We have finalized our implementation of this technology and Dr. Tapu Shaikh has submitted a manuscript that has been provisionally accepted for publication pending minor modifications.