HEMOCYANIN, COPPER CONTAINING OXYGEN TRANSPORT PROTEIN

血蓝蛋白、含铜氧运输蛋白

• 批准号: 6120514
• 负责人: KAREN A MAGNUS
• 金额: $ 0.02万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120514
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

Hemocyanins are oxygen transport proteins found in a variety of arthropods and mollusks. Hemocyanin from the common horseshoe crab has a molecular weight of about 3.3 million Da and is composed of 48 subunits each of 73 kDa. These subunits are eight different but related polypetides. Each subunit can reversibly bind one molecule of oxygen at an active site of two copper ions ligated by six histadine residues. The three dimensional structure of subunit II is know and available for molecular replacement phasing of the three new subunit crystal types collected at CHESS. In the 48 subunit whole hemocyanin molecule, each subunit type occupies only specific positions in the large macromolecular assembly. The subunit types differ significantly in their primary structures, ligand affinites, allosteric effectors and association properties, so comparing subunit structures will provide information about their individual functions as well as being a necessary prerequisite to understand the whole hemocyanin molecule in atomic detail.

血蓝蛋白是氧转运蛋白，存在于多种 节肢动物和软体动物。 鲎血蓝蛋白 分子量约为330万Da，由48个 每个亚基的分子量为73 kDa。 这些亚单位有八个不同的， 相关的息肉。 每个亚基可以可逆地结合一个分子的 六个组氨酸连接的两个铜离子的活性位点上的氧 残基 亚基II的三维结构是已知的， 可用于三个新亚基的分子置换定相 在国际象棋中收集的晶体类型。 在48亚基全血蓝蛋白中 分子中，每种亚基类型只占据特定的位置， 大分子组装体。 亚基类型差异显著 在它们的一级结构中，配体亲合物，变构效应物 和缔合性质，所以比较亚基结构将 提供有关其各自职能的信息， 了解整个血蓝蛋白分子的必要前提 在原子细节。