IN VIVO MRM OF TRANSGEN ANIMAL MODEL OF CEREBRAL AMYLOID ANGIOPATHY: HEMORRHAGE

脑淀粉样血管病转基因动物模型的体内 MRM：出血

• 批准号: 6220744
• 负责人: PATRICK M SULLIVAN
• 金额: $ 0.38万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220744
• 关键词: biomedical resource; nuclear magnetic resonance spectroscopy; plants

The number of reports within the literature describing plant response to Al3+ exposure reflects a widespread and growing concern about the effects of Al3+ release. Release of this potentially toxic element can occur through several processes, including profound environmental disturbance such as coal mining or "acid rain". We propose to test the hypothesis that the a symbioticfungus Pisolithus tinctorius growing on pine roots will increase tolerance of seedlings to Al3+, and thus will increase plant survival following toxin exposure. New techniques including magnetic resonance imaging (MRI as is used for medical diagnostics) and NMR spectroscopy of intact plants will be used to assess the effect of the fungus and toxin exposure on root growth, turnover and architecture and chemistry. Ultrastructural changes and localization of Al and P will also be determined. Seedlings inoculated with P. tinctorius and planted on two mine sites will also be studied, with the goal of confirming a beneficial effect of the symbiotic fungus in promoting ecosystem restoration. Specific contributions include: 1) dynamic views of root growth, architecture and turnover as a function of Al3+ exposure and mycorrhizal colonization, 2) determination of Al chemistry within intact roots, 3) determination of the effects of Al3+ on P metabolism 4) ultrastructural information and elemental localization, 5) correlative information from field trees, 6) confirmation of the role of P. tinctorius in providing protection to the host against Al3+ toxicity, and 7) further development of 27Al NMR spectroscopic technology which can be extended to additional studies.

描述植物的文献中的报告数量 对 Al3+ 暴露的反应反映了人们广泛且日益增长的担忧 关于 Al3+ 释放的影响。 这种潜在有毒物质的释放 元素可以通过几个过程发生，包括深刻的 环境干扰，例如煤矿开采或“酸雨”。 我们 提议检验共生真菌 Pisolithus 的假设 生长在松根上的tinctorius会增加幼苗的耐受性 Al3+，因此会增加植物在毒素后的存活率 接触。 新技术包括磁共振成像（MRI 用于医疗诊断）和完整植物的核磁共振波谱 将用于评估真菌和毒素暴露对 根系生长、更新、结构和化学。 超微结构 Al和P的变化和定位也将被确定。 接种 P.tinctorius 的幼苗并种植在两个矿区 还将进行研究，目的是确认有益效果 共生真菌在促进生态系统恢复中的作用。 具体的 贡献包括：1）根系生长、结构的动态视图 和周转率与 Al3+ 暴露和菌根的函数关系 定植，2) 测定完整根内的铝化学成分，3) Al3+对磷代谢影响的测定4) 超微结构信息和元素定位，5）相关 来自田间树木的信息，6）确认 P 的作用。 Tinctorius 为宿主提供保护以抵抗 Al3+ 毒性， 7)进一步发展27Al核磁共振波谱技术 可以扩展到其他研究。