A HUMAN APOE MOUSE MODEL OF ALZHEIMER?S DISEASE

阿尔茨海默病的人猿小鼠模型

• 批准号: 8171625
• 负责人: PATRICK M SULLIVAN
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171625
• 关键词: Alzheimer' s Disease; Alzheimer' s disease model; Animals; Apolipoprotein E; Behavioral; Brain; Brain imaging; Cerebral hemisphere hemorrhage; Computer Retrieval of Information on Scientific Projects Database; Dementia; Functional disorder; Funding; Gliosis; Grant; Human; Institution; Lateral; Life; Mus; Research; Research Personnel; Resources; Source; Synapses; United States National Institutes of Health; aged; apolipoprotein E-3; apolipoprotein E-4; base; lateral ventricle; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mice involved in this project are 21-23 month old human apoE3 and E4 targeted replacement mice on a C57Bl6 background. We have observed enlarged lateral ventricles in the brains of 18 mo. old human apoE4 mice (compared to apoE3 mice) based on histological sections and would like to confirm using brain imaging on live animals. We have several other indicators of Alzheimer's-like dementia in the aged apoE4 mice such as behavioral and electrophysiological deficits. Furthermore, the apoE4 mice have significantly higher number of intracerebral hemorrhages, gliosis and immunocytochemical markers of synaptic dysfunction. If we are able to demonstrate significant enlargement of the lateral (or other) ventricles in these mice vs controls, it would be a very important discovery, strengthening the evidence for these mice to be used as models of AD.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 参与该项目的小鼠是21-23月龄的人apoE 3和E4靶向的C57 B16背景的替代小鼠。我们观察到18个月大的侧脑室扩大。老年人apoE 4小鼠（与apoE 3小鼠相比）基于组织学切片，并希望使用活体动物的脑成像进行确认。 我们在老年apoE 4小鼠中有几个阿尔茨海默氏样痴呆的其他指标，如行为和 电生理缺陷 此外，apoE 4小鼠具有显著更高数量的脑内肿胀、神经胶质增生和突触功能障碍的免疫细胞化学标志物。 如果我们能够证明这些小鼠的侧脑室（或其他脑室）与对照组相比显着增大，这将是一个非常重要的发现，加强了这些小鼠用作AD模型的证据。