ANALYSIS OF PROTEIN TYROSINE SULFATION

蛋白质酪氨酸硫酸化分析

• 批准号: 6282461
• 负责人: GRACE L ROSENQUIST
• 金额: $ 1.17万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282461
• 关键词: Chordata; Mammalia; biomedical resource; cardiovascular system; connective tissue; enzymes; hematology; hormones; intestines; nervous system; proteins; stomach

Sulfation is a widespread post-translational modification of tyrosines found in all animal cells containing a Golgi system. In fact, approximately 1% of the tyrosines are sulfated in tissue in the rat. Despite this observation, only 24 unique protein sequences containing tyrosine sulfate have been described. The aim of this project is to analyze the amino acid sequences surrounding know tyrosine sulfation sites to determine the characteristics of this site and, therefore, to identify more candidates of sulfation. The specific goals are: 1. to identify the characteristics of sulfated tyrosines which distinguish them from non-sulfated tyrosines. These characteristics include the number and distribution of amino acids, as well as the possible secondary structural features in the vicinity of the tyrosine. 2. to identify proteins with probable tyrosine sulfation sites from the Swiss Protein data base. 3. to determine sequence similarities among the set of known proteins in the vicinity of the sulfation site. Identification of new protein sulfation sites may be essential for the understanding of the function of these proteins and will lead to a better understanding of the function of tyrosine sulfation.

硫酸化是一种广泛的翻译后修饰 酪氨酸存在于所有含有高尔基体系统的动物细胞中。 在 事实上，大约 1% 的酪氨酸在组织中被硫酸化。 鼠。 尽管有这样的观察结果，只有 24 个独特的蛋白质序列 已经描述了含有酪氨酸硫酸盐。 此举的目的 项目是分析已知的氨基酸序列 酪氨酸硫酸化位点以确定该位点的特征 因此，需要鉴定更多的硫酸化候选者。 这 具体目标是： 1. 确定硫酸盐的特性 酪氨酸将其与非硫酸化酪氨酸区分开来。 这些 特征包括氨基酸的数量和分布，如 以及附近可能的二级结构特征 酪氨酸。 2. 鉴定可能含有酪氨酸的蛋白质 来自瑞士蛋白质数据库的硫酸化位点。 3. 确定 附近已知蛋白质组之间的序列相似性 硫酸化位点。 新蛋白质硫酸化位点的鉴定 对于理解这些功能可能至关重要 蛋白质，将有助于更好地理解蛋白质的功能 酪氨酸硫酸化。