ADAPTATION OF EPR INSTRUMENTATION FOR USE IN HUMAN SUBJECTS

EPR 仪器的改造用于人体受试者

• 批准号: 6206512
• 负责人: TADEUSZ WALCZAK
• 金额: $ 0.61万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206512
• 关键词: Mammalia; bioengineering /biomedical engineering; biomaterials; biomedical resource; gas; medical rehabilitation related tag; neoplasm /cancer; radiology; rehabilitation; technology /technique

Due to the severe and untreatable consequences of radiation myelopathy, many tumors in the paraspinal region receive a less than optimal radiation dose. Even a modest reduction in the risk of radiation injury from a given therapeutic dose would allow for the use of an increased dose which could enhance the probability of a local cure. The ability to accurately assess oxygen concentration in the radiated spinal cord is critical to studying the underlying mechanisms of radiation myelopathy, and in helping to determine more effective ways to use and deliver radiation to cancers in the paraspinal region. Our results show that spinal cord tissue pO2 in the irradiated rats increased significantly at intermediate (maximum level of 18 q 3 mm Hg at 55 days post irradiation) and long time periods after the radiation (average level 15 q 3 mm Hg between 90 and 180 days post irradiation), compared with the non-irradiated control group (average level of 9 q 2 mm Hg throughout the experiment). The finding that the pO2 was elevated in the irradiated spinal cord was unexpected and suggests that the prevailing expectations on the pathophysiology of radiation-induced damage to the spinal cord should be reconsidered. It seems possible that the pathogenesis is related to oxidative damage associated with the increased pO2. Although the mechanisms for the increase in pO2 in this study are not currently known, decreased oxygen consumption and/or radiation induced loss of vascular tone and microvascular remodeling and dilatation in the irradiated region are plausible factors. These results also indicate that EPR oximetry is a useful and noninvasive method for repeatedly assessing the pO2 of CNS tissue non-invasively over an extended period of time.

由于辐射的严重和无法治愈的后果 脊髓病，许多肿瘤在脊柱旁区域接受不到 最佳辐射剂量 即使是轻微的降低 给定治疗剂量的辐射损伤将允许使用 增加剂量，这可能会增加局部 疗方 准确评估氧气浓度的能力 放射性脊髓对于研究其潜在机制至关重要 放射性脊髓病，并帮助确定更有效的 如何使用和提供放射治疗脊柱旁区域的癌症。 结果表明，照射后大鼠脊髓组织pO 2 在中等水平（18 q 3 mm Hg的最大水平）显著增加 照射后55天）和照射后长时间 （辐照后90至180天的平均水平为15 q 3 mm Hg）， 与未照射对照组（9 q2平均水平）比较 mmHg）。 结果表明，pO 2 在受辐射的脊髓中升高是出乎意料的， 人们普遍认为， 应该重新考虑辐射引起的脊髓损伤。 其发病机制可能与氧化损伤有关 与pO 2升高有关。 虽然机制， 本研究中pO 2增加目前未知， 氧消耗和/或辐射诱导的血管张力丧失， 微血管重塑和扩张在照射区域是 可能的因素 这些结果还表明，EPR血氧测定法是一种 一种可重复评估CNS pO 2的有用且无创的方法 在较长的时间段内非侵入性地对组织进行治疗。