ADAPTATION OF EPR INSTRUMENTATION FOR USE IN HUMAN SUBJECTS

EPR 仪器的改造用于人体受试者

• 批准号: 6353186
• 负责人: TADEUSZ WALCZAK
• 金额: $ 0.61万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353186
• 关键词: Mammalia; bioengineering /biomedical engineering; biomaterials; biomedical resource; gas; medical rehabilitation related tag; neoplasm /cancer; radiology; rehabilitation; technology /technique

Due to the severe and untreatable consequences of radiation myelopathy, many tumors in the paraspinal region receive a less than optimal radiation dose. Even a modest reduction in the risk of radiation injury from a given therapeutic dose would allow for the use of an increased dose which could enhance the probability of a local cure. The ability to accurately assess oxygen concentration in the radiated spinal cord is critical to studying the underlying mechanisms of radiation myelopathy, and in helping to determine more effective ways to use and deliver radiation to cancers in the paraspinal region. Our results show that spinal cord tissue pO2 in the irradiated rats increased significantly at intermediate (maximum level of 18 q 3 mm Hg at 55 days post irradiation) and long time periods after the radiation (average level 15 q 3 mm Hg between 90 and 180 days post irradiation), compared with the non-irradiated control group (average level of 9 q 2 mm Hg throughout the experiment). The finding that the pO2 was elevated in the irradiated spinal cord was unexpected and suggests that the prevailing expectations on the pathophysiology of radiation-induced damage to the spinal cord should be reconsidered. It seems possible that the pathogenesis is related to oxidative damage associated with the increased pO2. Although the mechanisms for the increase in pO2 in this study are not currently known, decreased oxygen consumption and/or radiation induced loss of vascular tone and microvascular remodeling and dilatation in the irradiated region are plausible factors. These results also indicate that EPR oximetry is a useful and noninvasive method for repeatedly assessing the pO2 of CNS tissue non-invasively over an extended period of time.

由于辐射的严重且无法治疗的后果 脊髓病，许多椎旁区域的肿瘤接受的治疗少于 最佳辐射剂量。 即使风险略有降低 给定治疗剂量的辐射损伤将允许使用 增加剂量可能会增加局部感染的可能性 治愈。 能够准确评估环境中的氧气浓度 辐射脊髓对于研究潜在机制至关重要 放射性脊髓病，并帮助确定更有效的 使用放射线治疗椎旁区域癌症的方法。 我们的结果表明，受辐射大鼠的脊髓组织 pO2 中间显着增加（最高水平为 18 q 3 mm Hg 照射后 55 天）和照射后很长一段时间 （照射后 90 至 180 天的平均水平为 15 q 3 mm Hg）， 与未照射对照组相比（平均水平为 9 q 2 整个实验过程中的毫米汞柱）。 结果发现 pO2 为 受辐射脊髓中的升高是出乎意料的，表明 人们对病理生理学的普遍期望 应重新考虑辐射引起的脊髓损伤。 发病机制似乎可能与氧化损伤有关 与 pO2 增加有关。 尽管机制 本研究中 pO2 的增加目前未知，减少 耗氧量和/或辐射引起的血管张力丧失和 照射区域的微血管重塑和扩张 合理的因素。 这些结果还表明 EPR 血氧测定法是一种 重复评估 CNS 氧分压 (pO2) 的有用且无创的方法 长时间内以非侵入性方式保护组织。