A GENOME SCAN FOR SUSCEPTIBILITY TO HELMINTHIC INFECTION

描述(逐字摘自申请者摘要)：蠕虫感染 世界热带和温带地区的主要公共卫生问题。 令人振奋的新证据表明，遗传因素可能在 确定其中一些疾病对感染的易感性。这 该项目提出了一种基因组扫描来检测和定位特定的基因 对钩虫、蛔虫和鞭虫感染易感性的影响 属于吉雷尔单一最大家系的1205个个体 尼泊尔东部的人口。以15年的基因研究为基础 吉雷尔种群，包括三年的遗传研究 肠道蠕虫感染的流行病学，这个项目将利用 优秀的现有蠕虫感染数据、血液数据库和资源 样本和系谱信息，以执行第一个大规模基因组 扫描影响这些重要疾病易感性的基因。我们会 使用自动基因分型技术每隔10厘米放置STR标记 基因组。然后我们将使用最先进的链接分析方法来检测 影响蠕虫感染相关数量表型的基因 包括从粪便样本中确定的初始卵数和蠕虫数 在阿苯达唑治疗钩虫后96小时内收集， 蛔虫和鞭虫。确定了这些蠕虫感染的表型。 在两个时间点，在最初的调查中和在1 在第一次调查之后的一年。该项目将提供有关以下方面的新信息 肠道蠕虫感染易感性的遗传决定因素。

DESCRIPTION (Verbatim From Applicant's Abstract): Helminthic infections are major public health concerns in both tropical and temperate areas of the world. Exciting new evidence suggests that genetic factors may play important roles in determining susceptibility to infection for a number of these diseases. This project proposes a genomic scan to detect and localize the specific genes that influence susceptibility to infection with hookworm, roundworm, and whipworm in 1205 individuals belonging to the single largest pedigree of the Jirel population of eastern Nepal. Building upon 15 years of genetic research with the Jirel population, including three years of research on the genetic epidemiology of intestinal worm infections, this project will utilize the outstanding existing database and resource of helminthic infection data, blood samples, and pedigree information, to perform the first large-scale genomic scan for genes influencing susceptibility to these important diseases. We will use automated genotyping techniques to place STR markers every 10cM across the genome. We will then use state-of-the-art linkage analysis methods to detect genes influencing quantitative phenotypes associated with helminthic infection including initial egg counts and worm counts determined from stool samples collected for 96 hours following treatment with albendazole for hookworm, roundworm and whipworm. These helminthic infection phenotypes were determined at two points in time, in an initial survey and in a follow-up sample from 1 year after the initial survey. This project will provide new information about the genetic determinants of susceptibility to intestinal worm infections.