An Airyscan 2 confocal laser scanning microscope for the Wolfson Light Microscopy Facility

适用于 Wolfson 光学显微镜设备的 Airyscan 2 共焦激光扫描显微镜

• 批准号: MR/X012077/1
• 负责人: Darren Robinson
• 金额: $ 70.81万
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012077%2F1
• 关键词: Airyscan; confocal; laser; scanning; microscope

Human diseases arise because of defects in fundamental biological processes. These processes depend on interactions between molecules within cells, between cells within tissues and tissues in the whole organism. In order to understand and identify effective therapeutics we need to understand the dynamic nature of biological processes and observing such processes in living cells is fundamental to this understanding. Visualisation both validates other experimental approaches but, importantly, often allows us to formulate new hypotheses. At the University of Sheffield biomedical researchers are exploring the fundamental way in which molecules, cells and tissues behave in order to understand what goes wrong in diseases such as cancer, infection, inflammation, deafness and neurodegeneration. In order to observe biological processes we often attach fluorescent labels to components of interest and follow their behaviour using fluorescence microscopes. The time-scale of biological processes is highly variable: some processes happen very quickly, meaning that images need to be acquired speedily while others occur over a much longer time-scale of minutes to hours. There are a number of challenges associated with capturing fluorescence images because the exposure of cells to light can cause phototoxicity which damages cells and causes aberrant behaviour. Additionally, some fluorescent labels can be quite dim which makes the fluorescent signal difficult to detect. The aim of this proposal is to obtain funding for the latest Airyscan 2 confocal laser scanning microscope (CLSM). This microscope will not only replace the functionality of two existing but very old fluorescent microscopes which are difficult to maintain, but will also improve our ability to observe very small structures within cells and/or to observe highly dynamic processes which occur very rapidly. The advanced performance of the Airyscan 2 CLSM will overcome current limitations in understanding fundamental mechanisms that underpin disease.

人类疾病是由于基本生物过程中的缺陷而产生的。这些过程依赖于细胞内分子之间、组织内细胞之间和整个有机体组织之间的相互作用。为了理解和确定有效的治疗方法，我们需要了解生物过程的动态性质，而观察活细胞中的这种过程是理解这一过程的基础。形象化既验证了其他实验方法，但重要的是，它往往允许我们提出新的假设。谢菲尔德大学的生物医学研究人员正在探索分子、细胞和组织的基本行为方式，以了解癌症、感染、炎症、耳聋和神经退化等疾病的问题所在。为了观察生物过程，我们经常在感兴趣的成分上贴上荧光标记，并使用荧光显微镜跟踪它们的行为。生物过程的时间尺度是高度可变的：一些过程发生得非常快，这意味着需要快速获取图像，而另一些过程则发生在更长的时间尺度上--几分钟到几小时。捕捉荧光图像有许多挑战，因为细胞暴露在光下会引起光毒性，这会损害细胞并导致异常行为。此外，一些荧光标签可能非常暗，这使得荧光信号很难被检测到。这项提案的目的是为最新的AiryScan 2共焦激光扫描显微镜(CLSM)获得资金。这种显微镜不仅将取代现有的两种难以维护的非常老旧的荧光显微镜的功能，而且还将提高我们观察细胞内非常微小的结构和/或观察非常快速发生的高度动态过程的能力。AiryScan 2 CLSM的先进性能将克服目前在理解疾病基础机制方面的局限性。

项目成果

Oncogenic Ras deregulates cell-substrate interactions during mitotic rounding and respreading to alter cell division orientation.

• DOI: 10.1016/j.cub.2023.05.061
• 发表时间: 2023-07-10
• 期刊: Current biology : CB
• 作者: Ganguli S;Wyatt T;Nyga A;Lawson RH;Meyer T;Baum B;Matthews HK
• 通讯作者: Matthews HK

Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides

• DOI: 10.1101/2023.10.18.562882
• 发表时间: 2023-10
• 期刊: The Journal of Cell Biology
• 作者: Hannes Maib;Petia Adarska;Robert Hunton;James H Vines;David Strutt;Francesca Bottanelli;David H. Murray

Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy.

• DOI: 10.1039/d3nr01129a
• 发表时间: 2023-11-23
• 期刊: NANOSCALE
• 影响因子: 6.7
• 作者: Sheard, Thomas M. D.;Shakespeare, Tayla B.;Seehra, Rajpinder S.;Spencer, Michael E.;Suen, Kin M.;Jayasinghe, Izzy
• 通讯作者: Jayasinghe, Izzy