Modernising the Electron Microscopy capabilities at the University of Glasgow

格拉斯哥大学电子显微镜能力的现代化

• 批准号: MR/X013073/1
• 负责人: Leandro Lemgruber
• 金额: $ 78.33万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013073%2F1
• 关键词: Modernising; Electron; Microscopy; capabilities; University

The College of Medical, Veterinary & Life Sciences at the University in Glasgow hosts world-leading research and is a major hub for studies relevant to human health, ranging from fundamental science to nanomedicine, through pathogen and infection research. To maintain this high-quality environment, the College has recently created an integrated Imaging Facility, improving management of and training on diverse imaging systems (both light and electron microscope based) located at the Gilmorehill campus. An up-to-date facility will contribute to the continuous successful outputs, while providing researchers with access to world-class equipment supported by experienced technologists. We seek funds to replace a 35 years of use transmission electron microscope, which will allow to improve and expand the imaging capacities in the facility. Several current projects that span the remit of MRC strategic plans are at a stage requiring techniques that cannot be fulfilled by the current instrumentation available at the facility. The upgrade of our capabilities in electron microscopy ensures that the facility offers substantially better services.In this proposal we have highlighted some projects (led by Early Careers Researchers to mid-careers and full Professors) that would benefit from the requested up-to-date TEM, spanning from studies of infection and immunity to molecular and cellular medicine, through neuroscience and population medicine and global health. A modern TEM would also allow the facility to continue to train new generations of skilled researchers with specific expertise, through training and support in the use of the microscope.The immediate scientific outcomes from the new equipment will also have long-term benefits for the Glasgow scientific community, providing incentives for further project bids and fostering cooperation between research teams. This will be particularly valuable for early career researchers, as it will support their projects by additions of key experimental techniques without the need of a prior large investment. It will also contribute to create a high-quality training and research environment, facilitating international collaborations and recruitment and, as such, benefiting the wider Scottish community. Such an environment will also benefit research students by providing access to valuable training and expanding their career options in the future. In view of these advantages, the College is committed to ensuring that the facility is optimally managed and that resources are provided for technical staff to maintain the equipment and support the users in their experiments. The equipment requested will be central for the new imaging facility, to incorporate it in the College strategy and support multiple users with the existing and future projects.

格拉斯哥大学的医学、兽医和生命科学学院拥有世界领先的研究，是与人类健康相关的研究的主要枢纽，从基础科学到纳米医学，再到病原体和感染研究。为了保持这种高质量的环境，学院最近创建了一个综合成像设施，改善了位于吉尔莫希尔校区的各种成像系统(基于光学和电子显微镜)的管理和培训。最新的设施将有助于持续成功的产出，同时为研究人员提供由经验丰富的技术人员支持的世界级设备。我们寻求资金来更换使用了35年的透射式电子显微镜，这将允许改善和扩大该设施的成像能力。目前几个跨越MRC战略计划范围的项目正处于需要技术的阶段，而该设施现有的仪器无法实现这些技术。我们在电子显微镜方面能力的升级确保了该设施提供更好的服务。在这份提案中，我们强调了一些项目(由早期职业研究人员领导到职业中期和全职教授)，这些项目将受益于所要求的最新的电子显微镜，范围从感染和免疫的研究到分子和细胞医学，通过神经科学和人口医学和全球健康。现代化的瞬变显微镜还将使该设施能够通过使用显微镜的培训和支持，继续培训具有特定专业知识的新一代熟练研究人员。新设备产生的直接科学成果也将为格拉斯哥科学界带来长期利益，为进一步的项目投标提供激励，并促进研究团队之间的合作。这将对早期职业研究人员特别有价值，因为它将通过增加关键实验技术来支持他们的项目，而不需要事先进行大量投资。它还将有助于创造一个高质量的培训和研究环境，促进国际合作和招聘，从而使更广泛的苏格兰社区受益。这样的环境也将使研究生受益，因为他们可以获得有价值的培训，并在未来扩大他们的职业选择。鉴于这些优势，学院致力于确保设施得到最佳管理，并为技术人员提供资源，以维护设备并支持用户进行实验。所需设备将是新成像设施的核心，以便将其纳入学院战略，并通过现有和未来的项目支持多个用户。

项目成果

Fractalkine enhances oligodendrocyte regeneration and remyelination in a demyelination mouse model.

• DOI: 10.1016/j.stemcr.2022.12.001
• 发表时间: 2023-02-14
• 期刊: STEM CELL REPORTS
• 影响因子: 5.9
• 作者: de Almeida, Monique M. A.;Watson, Adrianne E. S.;Bibi, Sana;Dittmann, Nicole L.;Goodkey, Kara;Sharafodinzadeh, Pedram;Galleguillos, Danny;Nakhaei-Nejad, Maryam;Kosaraju, Jayasankar;Steinberg, Noam;Wang, Beatrix S.;Footz, Tim;Giuliani, Fabrizio;Wang, Jing;Sipione, Simonetta;Edgar, Julia M.;Voronova, Anastassia
• 通讯作者: Voronova, Anastassia

Digitizing protocols into single reactors for the one-pot synthesis of nanomaterials

• DOI: 10.1016/j.matt.2023.05.029
• 发表时间: 2023-07
• 期刊: Matter
• 影响因子: 18.9
• 作者: Hsin-Herng Wang;J. S. Manzano;Philip J. Kitson;M. Mullin;Chang-Gen Lin;Igor I. Slowing;L. Cronin

Structure of the Saccharolobus solfataricus type III-D CRISPR effector.

• DOI: 10.1016/j.crstbi.2023.100098
• 发表时间: 2023
• 期刊: CURRENT RESEARCH IN STRUCTURAL BIOLOGY
• 影响因子: 2.8
• 作者: Cannone, Giuseppe;Kompaniiets, Dmytro;Graham, Shirley;White, Malcolm F;Spagnolo, Laura
• 通讯作者: Spagnolo, Laura

Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function

• DOI: 10.1101/2023.01.13.523951
• 发表时间: 2023-01
• 期刊: bioRxiv
• 作者: Alice Santi;Emily J. Kay;L. Neilson;L. Mcgarry;S. Lilla;Margaret Mullin;Nikki R. Paul;F. Fercoq;Grigorios Koulouras;G. R. Blanco;D. Athineos;Susan Mason;Mark Hughes;Y. Kieffer;Colin Nixon;K. Blyth;F. Mechta-Grigoriou;Leo M. Carlin;S. Zanivan

Transferrin-Bearing, Zein-Based Hybrid Lipid Nanoparticles for Drug and Gene Delivery to Prostate Cancer Cells.

• DOI: 10.3390/pharmaceutics15112643
• 发表时间: 2023-11-20
• 期刊: Pharmaceutics
• 影响因子: 5.4
• 作者: Maeyouf K;Sakpakdeejaroen I;Somani S;Meewan J;Ali-Jerman H;Laskar P;Mullin M;MacKenzie G;Tate RJ;Dufès C
• 通讯作者: Dufès C