Provision of a MALDI-MSI Platform for Biomedical Analytical Imaging

提供用于生物医学分析成像的 MALDI-MSI 平台

• 批准号: MR/X013553/1
• 负责人: Andrew Bottrill
• 金额: $ 22.05万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013553%2F1
• 关键词: Provision; MALDI; MSI; Platform; Biomedical

Mass spectrometry (MS) is a powerful analytical technique that is widely used to identify compounds from a vast array of materials and to study biological molecules and relate them to function. Frequently we compare samples to understand the differences between normal and diseased or treated conditions, for example healthy tissue compared with tissue infected with a naturally occurring pathogen, for example bacteria or a virus. Understanding the molecular responses to infection are key to implementing treatments.To carry out MS experiments, samples such as bacteria or cells are normally broken down as a whole and compounds extracted for analysis. Whilst extremely sensitive at identifying the molecules present, this method loses the spatial location of the compounds; they may be distributed differently in different areas.Mass spectrometry imaging (MSI) is a technique that allows characterisation of biological molecules across surfaces. A laser is fired at a very small area on the surface of a sample to generate charged molecules (called ions) from that position, and these are analysed by the mass spectrometer. The sample is moved very slightly, and the laser fired again. In this way we can detected analytes across the surface and build up an image-map. This will allow us to relate the position of biological molecules to function and will be a great improvement on the equipment already available to us.We propose to use this equipment undertake projects aligned with MRC research portfolio and priorities in themes such as antimicrobial resistance, immunity and infection through the life course, and neurodegeneration supporting existing MRC projects and planned MRC submissions. The project will also support the development of research technical professionals.

质谱 (MS) 是一种强大的分析技术，广泛用于从大量材料中识别化合物、研究生物分子并将其与功能联系起来。我们经常比较样本，以了解正常和患病或治疗条件之间的差异，例如健康组织与受自然发生的病原体（例如细菌或病毒）感染的组织相比。了解对感染的分子反应是实施治疗的关键。为了进行质谱实验，通常将细菌或细胞等样品作为一个整体进行分解，并提取化合物进行分析。虽然这种方法在识别存在的分子方面极其灵敏，但会丢失化合物的空间位置。它们在不同区域的分布可能不同。质谱成像 (MSI) 是一种可以对表面上的生物分子进行表征的技术。激光发射到样品表面的一个非常小的区域，从该位置产生带电分子（称为离子），并由质谱仪对这些分子进行分析。样品稍微移动，激光再次发射。通过这种方式，我们可以检测整个表面的分析物并建立图像图。这将使我们能够将生物分子的位置与功能联系起来，并将对我们现有的设备进行重大改进。我们建议使用该设备开展与 MRC 研究组合和主题优先事项一致的项目，例如抗菌素耐药性、整个生命过程中的免疫和感染以及神经退行性疾病，支持现有的 MRC 项目和计划的 MRC 提交。该项目还将支持研究技术专业人员的发展。