MALDI-TOF/TOF Mass spectrometer

MALDI-TOF/TOF质谱仪

• 批准号: 528773185
• 金额: --
• 依托单位: Universität Ulm
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/528773185?language=en
• 关键词: MALDI; TOF; Mass; spectrometer

MALDI mass spectrometry is the optimal ionization method for large and nonpolar molecules and for this reason essential for several research groups of the Chemical Institutes of the University of Ulm. The instrument, which is currently centrally operated at the Mass Spectrometry Service Center and is very well utilized, was purchased in 1998. The analytical services performed on this instrument include identity control of organic polymers and macrocycles, metal complexes, peptides, glycans and oligonucleotides, identification of proteins from 1D and 2D gel bands and localization of post-translational modifications by "peptide mapping". These services are available to all research groups within the Faculty of Natural Sciences of the University of Ulm within the framework of the user regulations. The instrument is without alternative for measurements in the high mass range (> 10,000 Da), measurements with high sample throughput and as a "backup instrument" for the high-resolution FTICR instrument. Recently, however, downtime and repairs have been accumulating due to aged electronic components caused by the long lifetime of the instrument. Support from the manufacturer was discontinued in 2014, and repairs must be performed in-house with a limited number of spare parts still available. Due to high equipment downtime and limited functionality, reliable and continuous service operation is no longer possible. Therefore, financial support for a replacement by a new more powerful MALDI-TOF/TOF instrument is hereby requested.

MALDI质谱仪是大分子和非极性分子的最佳电离方法，因此对于乌尔姆大学化学研究所的几个研究小组来说是必不可少的。该仪器是1998年购买的，目前在质谱学服务中心集中操作，并得到了很好的利用。在这台仪器上进行的分析服务包括有机聚合物和大环、金属络合物、多肽、多糖和寡核苷酸的身份控制，从一维和二维凝胶条带识别蛋白质，以及通过“肽图”定位翻译后修饰。在《用户条例》的框架内，乌尔姆大学自然科学学院内的所有研究小组都可获得这些服务。该仪器对于高质量范围(&gt；10,000 Da)的测量、高样品吞吐量的测量以及高分辨率FTICR仪器的“备份仪器”都是不可替代的。然而，最近，由于仪器寿命长而导致电子元件老化，停机和维修一直在积累。制造商的支持在2014年停止，维修必须在仍有有限数量的备件的情况下在内部进行。由于设备停机时间长，功能有限，不再可能进行可靠和连续的服务操作。因此，现请求提供资金支持，以取代功能更强大的新型MALDI-TOF/TOF仪器。