GENETIC STUDIES OF DIABETIC NEPHROPATHY

描述：这是一项研究，旨在确定糖尿病肾病 (DN)在基因上与一组建议的候选基因中的任何一个相关联 它在肾脏中的功能表明它在遗传易感性中发挥了作用， 并使用微阵列技术来识别差异基因 在糖尿病肾病和非糖尿病肾病患者的肾脏中均有表达。基因的cDNA 候选基因，以及来自肾脏cDNA文库的随机克隆，将 接受筛查。以前确定的候选基因和其他已知基因 不被认为是候选人的人，将通过这次测试来确定 差异表达。也有可能是以前未知的基因 将以这种方式被识别。所有这些基因都将被赋予高度的 在这些测试中，优先考虑和额外检查与糖尿病肾病的联系。vbl.使用 这些基因及其附近区域的每一个的序列信息， 基因内和附近的多态将被识别出来。测试，测试 调查人员正在组建一个家庭小组，这些家庭有 几个具有DN的成员，或以其他方式适合于连锁分析。 将进行连杆不平衡测试(使用 传输/不平衡测试，以及在可能的情况下，受影响的同胞 配对和其他同胞配对检验方法。

DESCRIPTION: This is a study to determine whether diabetic nephropathy (DN) is genetically linked to any of a set of proposed candidate gene whose function in the kidney suggests a role in genetic susceptibility, and to use microarray technology to identify genes that are differentially expressed in the kidneys of diabetics with and without DN. cDNAs for candidate genes, as well as random clones from a kidney cDNA library, will be screened. Previously identified candidate genes, and other known genes that were not considered candidates, will be identified by this test for differential expression. It is also possible that previously unknown genes will be identified in this way. All of these genes will be given high priority and extra scrutiny in these tests for linkage with DN. Using sequence information for each of these genes and nearby regions, polymorphisms in and near the genes will be identified. To test for linkage, the investigators are assembling a panel of families that have several members with DN, or are otherwise suitable for linkage analysis. Tests will be carried out for linkage disequilibrium (using the transmission/disequilibrium test, TDT) and where possible, affected sib pair and other sib pair test methods.