VAGAL MEDIATED CYTOKINES IN SLEEP CIRCUITRY

睡眠回路中迷走神经介导的细胞因子

• 批准号: 6133347
• 负责人: LYNN D CHURCHILL
• 金额: $ 7.14万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6133347
• 关键词: NMDA receptors; Rodentias; adrenergic agents; afferent nerve; arousal; brain stem; cytokine; hypothalamus; inhibitor /antagonist; interleukin 1; microinjections; neuropharmacology; parabrachial nucleus; preoptic areas; procaine; propranolol; quinolinate; sleep; sleep regulatory center; solitary tract nucleus; tumor necrosis factor alpha; vagus nerve

DESCRIPTION (adapted from applicant's abstract): Excessive food intake and peripheral infectious challenge are associated with increases in non-rapid eye movement sleep (NREMS). The vagus and vagal-induced upregulation of cytokines seems to mediate these sleep responses, in part. However, whether action potentials in the vagus induce cytokine upregulation in the brain and the central circuit tying vagal afferents to increased sleep and cytokine production remains unknown. Vagal-mediated intracerebral increases in cytokine mRNA levels have been observed in the brainstem and hypothalamus, two areas implicated in regulating NREMS. Neuroanatomical localization of the cell types involved in these increased cytokine mRNA levels are evaluated in a series of experimental tests. The following hypotheses are evaluated: (1) action potentials in vagal afferents are responsible and (2) the neural circuitry from the vagal afferent projection to the nucleus of the solitary tract through the lateral parabrachial nucleus to the anterior hypothalamus and medial preoptic area is responsible. Finally, the hypotheses that neurons within this circuitry are upregulating their cytokine mRNA levels in a vagal-mediated fashion is tested by retrogradely labeling specific neural projections. Mapping the vagal-mediated intracerebral cytokines within the brainstem and hypothalamus will provide new insights into the functional neuroanatomy that underlies the regulation of NREMS.

描述(摘自申请者摘要)：食物摄入量过多和 外周感染性挑战与非快速眼球增多相关 运动睡眠(NREMS)。迷走神经及迷走神经诱导的细胞因子上调 似乎在一定程度上调节了这些睡眠反应。然而，无论是采取行动 迷走神经中的电位诱导脑内细胞因子上调 迷走神经传入与睡眠和细胞因子增加有关的中枢回路 产量仍不得而知。迷走神经介导的脑内细胞因子增加 在脑干和下丘脑这两个区域观察到了mrna的表达。 牵涉到对NREMS的监管。细胞类型的神经解剖学定位 参与这些细胞因子mRNA水平增加的一系列评估 实验测试。评估了以下假设：(1)行动 迷走神经传入中的电位是相关的，(2)来自 迷走神经在孤束核的传入投射 臂旁外侧核至下丘脑前部和视前内侧 地区要对此负责。最后，假设这个回路中的神经元 正在以迷走神经介导的方式上调他们的细胞因子mRNA水平 通过逆行标记特定的神经投射进行测试。映射 脑干和下丘脑内迷走神经介导的脑内细胞因子 将提供对功能神经解剖学的新见解， 对NREMS的监管。