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VAGAL-MEDIATED CYTOKINES IN SLEEP CIRCUITRY

睡眠回路中迷走神经介导的细胞因子

基本信息

批准号：
6392627
负责人：
LYNN D CHURCHILL
金额：
$ 7.14万
依托单位：
WASHINGTON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-05-01 至 2003-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (adapted from applicant's abstract): Excessive food intake and peripheral infectious challenge are associated with increases in non-rapid eye movement sleep (NREMS). The vagus and vagal-induced upregulation of cytokines seems to mediate these sleep responses, in part. However, whether action potentials in the vagus induce cytokine upregulation in the brain and the central circuit tying vagal afferents to increased sleep and cytokine production remains unknown. Vagal-mediated intracerebral increases in cytokine mRNA levels have been observed in the brainstem and hypothalamus, two areas implicated in regulating NREMS. Neuroanatomical localization of the cell types involved in these increased cytokine mRNA levels are evaluated in a series of experimental tests. The following hypotheses are evaluated: (1) action potentials in vagal afferents are responsible and (2) the neural circuitry from the vagal afferent projection to the nucleus of the solitary tract through the lateral parabrachial nucleus to the anterior hypothalamus and medial preoptic area is responsible. Finally, the hypotheses that neurons within this circuitry are upregulating their cytokine mRNA levels in a vagal-mediated fashion is tested by retrogradely labeling specific neural projections. Mapping the vagal-mediated intracerebral cytokines within the brainstem and hypothalamus will provide new insights into the functional neuroanatomy that underlies the regulation of NREMS.

描述（改编自申请人的摘要）：食物摄入过多和外周感染挑战与非快速眼病的增加有关运动睡眠（NREMS）。迷走神经和迷走神经诱导的细胞因子上调似乎在一定程度上调节了这些睡眠反应。然而，无论行动迷走神经的电位诱导大脑中细胞因子的上调中枢回路将迷走神经传入与增加的睡眠和细胞因子联系起来产量仍未知。迷走神经介导的脑内细胞因子增加在脑干和下丘脑这两个区域中观察到 mRNA 水平参与监管 NREMS。细胞类型的神经解剖学定位参与这些增加的细胞因子 mRNA 水平的评估通过一系列实验测试。评估以下假设： (1) 行动迷走神经传入神经的电位负责，（2）神经回路迷走神经传入投射到孤束核外侧臂旁核至下丘脑前部和内侧视前区区负责。最后，假设该电路中的神经元以迷走神经介导的方式上调细胞因子 mRNA 水平通过逆行标记特定的神经投射进行测试。映射脑干和下丘脑内迷走神经介导的脑内细胞因子将为功能神经解剖学提供新的见解 NREMS 的监管。