MECHANISMS OF STRESS SPECIFIC CHANGES IN GENE EXPRESSION

基因表达中应激特异性变化的机制

• 批准号: 6188772
• 负责人: Esther Louise Sabban
• 金额: $ 3.1万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188772
• 关键词: DNA binding protein; adrenal medulla; cAMP response element binding protein; enzyme induction /repression; epinephrine; gel mobility shift assay; gene expression; genetic regulatory element; genetically modified animals; in situ hybridization; laboratory mouse; laboratory rat; locus coeruleus; neuroendocrine system; norepinephrine; stress; sympathetic ganglion; transcription factor; western blottings

DESCRIPTION Early responses to stress include activation of noradrenergic neurons of the locus coeruleus, and the sympathetic neuronal and adrenomedullary systems, resulting in production of the central and peripheral catecholamines norepinephrine and epinephrine. Stress also elevates gene expression for catecholamine biosynthetic enzymes, and there are gene-, tissue-, and stressor-specific differences in these responses to stress. The proposal investigates the mechanisms of regulation of gene expression of catecholamine biosynthetic enzymes (TH, DBH, and PNMT) in vivo in the adrenal medulla, sympathetic ganglia, and in different brain areas of rats exposed acutely, repeatedly or chronically to various stressors. Specific aims are: (1) determine effects of different stressors on TH, DBH, and PNMT gene expression, (2) determine dependence of single or repeated stress-elicited effects of TH, DBH, and PNMT expression on the transcription factor c-fos, (3) characterize the basis for differential regulation of TH and DBH gene expression in sympathetic ganglia compared to adrenal medulla, (4) determine effects of prior chronic or repeated exposure to stressors in the response to a novel stressor and (5) determine if TH and DBH gene expression is detectable in brain areas other than the major catecholaminergic cell fields, and if expression in these areas is regulated by stressors.

描述

项目成果

Identification of tyrosine hydroxylase gene expression in rat spleen.

大鼠脾脏中酪氨酸羟化酶基因表达的鉴定。

• DOI: 10.1016/s0304-3940(01)02126-7
• 发表时间: 2001
• 期刊: Neuroscience letters
• 影响因子: 2.5
• 作者: Kubovcáková,L;Micutková,L;Sabban,EL;Krizanová,O;Kvetnanský,R
• 通讯作者: Kvetnanský,R

Stress increases gene expression of phenylethanolamine N-methyltransferase in spleen of rats via pituitary-adrenocortical mechanism.

压力通过垂体-肾上腺皮质机制增加大鼠脾脏中苯乙醇胺 N-甲基转移酶的基因表达。

• DOI: 10.1016/s0306-4530(01)00098-1
• 发表时间: 2002
• 期刊: Psychoneuroendocrinology
• 影响因子: 3.7
• 作者: Jelokova,J;Rusnak,M;Kubovcakova,L;Buckendahl,P;Krizanova,O;Sabban,EL;Kvetnansky,R
• 通讯作者: Kvetnansky,R

Comparative study of catecholamine synthesizing enzymes in adrenal medulla of CRH knock-out mice, their CRH (+/+) mates and Sprague-Dawley rats.

CRH敲除小鼠、其CRH(/)伴侣和Sprague-Dawley大鼠肾上腺髓质儿茶酚胺合成酶的比较研究。

• 发表时间: 2002
• 期刊: Endocrine regulations.
• 作者: Kubovcakova,Lucia;Sabban,EstherL;Kvetnansky,Richard;Krizanova,Olga
• 通讯作者: Krizanova,Olga