CORE--PREPARATION, STORAGE, AND SHIPPING OF PLANT EXTRACTS AND ASSAYS

核心——植物提取物和检测的制备、储存和运输

• 批准号: 6352742
• 负责人: Sidney Michael Hecht
• 金额: $ 14.83万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352742
• 关键词: DNA damage; DNA directed DNA polymerase; DNA topoisomerases; antineoplastics; chromatography; drug screening /evaluation; enzyme inhibitors; plant extracts; preservation

This Core facility will be responsible for carrying out the preparation, storage and shipping of plant extracts, and much of the assay work run within the NPDDG. This is to include . soaking of plant materials; weighing, packaging, shipping and inventorying of plant extracts . testing of extracts for the presence of DNA damaging agents, DNA topoisomerase inhibitors and DNA polymerase beta inhibitors, in support of Programs 2. . isolation of purified DNA topoisomerase I and DNA polymerase beta for assay work . screening of samples received from NCI natural products repository . guiding the choice of extracts by the development/application of secondary discrimination assays in support of Laboratory Programs 2 . guiding the fractionation of extracts within Laboratory Program 3

该核心设施将负责进行准备， 植物提取物的存储和运输以及大部分测定工作运行 在NPDDG中。 这包括 。 浸泡植物材料；称重，包装，运输和 植物提取物的库存 。 测试提取物是否存在DNA损伤剂，DNA 拓扑异构酶抑制剂和DNA聚合酶β抑制剂，以支持 程序2。 。 纯化的DNA拓扑异构酶I和DNA聚合酶β的分离 测定工作 。 筛选从NCI天然产品存储库中收到的样品 。 通过开发/应用指导提取物的选择 支持实验室计划的次要歧视测定2 。 指导实验室计划中提取物的分馏3