RAMAN DETECTION OF HUMAN MACULAR CAROTENOID PIGMENTS

人黄斑类胡萝卜素色素的拉曼检测

• 批准号: 2720354
• 负责人: WERNER GELLERMANN
• 金额: $ 13.86万
• 依托单位: SPECTROTEK CORPORATION, LC
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2720354
• 关键词: Primates; Raman spectrometry; biomedical equipment development; carotenoids; charge coupled device camera; diagnosis design /evaluation; eye disorder diagnosis; fiber optics; holography; macular degeneration; macular drusen; retina

The goal of this project is to develop a novel, non-invasive laser-optical technology for early diagnosis of age. related macular degeneration (AMD), the leading cause of blindness in the elderly in the United States. The proposed development of a compact, low-cost diagnostic apparatus could ultimately provide the ophthalmologic and optometric communities with a powerful new screening test for AMD risk. The new technology uses Raman spectroscopy to detect the macular carotenoids lutein and zeaxanthin in the human retina. Recent basic and clinical science studies have indicated that these carotenoid compounds play a pivotal role in protecting the human macula from light, induced oxidative damage. Based on preliminary results, the Raman technology has the potential to provide a sensitive, fast, quantitative and objective optical detection of macular carotenoids. We propose to develop the Raman instrumentation, in a first phase, from a complex laboratory setup into a compact movable apparatus suitable for use on living humans and non-human primates. This involves the integration of a low-power laser, a holographic light dispersion element, light collection with a CCD array detector, and fiber-based light delivery and collection optics. PROPOSED COMMERCIAL APPLICATION: This technology has a high potential of leading to a novel non-invasive diagnostic instrument for screening and clinical studies of age, related macular degeneration. Potential users are practitioners in the ophthalmologic and optometric communities.

该项目的目标是开发一种新型的非侵入性激光光学技术，用于早期诊断年龄。相关性黄斑变性（AMD）是美国老年人失明的主要原因。拟议开发的紧凑型、低成本诊断设备最终可以为眼科和验光界提供强大的新型AMD风险筛查测试。这项新技术利用拉曼光谱检测人类视网膜中的黄斑类胡萝卜素、叶黄素和玉米黄质。最近的基础和临床科学研究表明，这些类胡萝卜素化合物在保护人类黄斑免受光诱导的氧化损伤方面起着关键作用。基于初步结果，拉曼技术具有提供黄斑类胡萝卜素的灵敏、快速、定量和客观的光学检测的潜力。我们建议开发的拉曼仪器，在第一阶段，从一个复杂的实验室设置成一个紧凑的可移动的设备，适用于活的人类和非人类灵长类动物。这涉及低功率激光器、全息光色散元件、具有CCD阵列检测器的光收集以及基于光纤的光传输和收集光学器件的集成。拟定商业应用：这项技术具有很高的潜力，导致一种新的非侵入性诊断仪器的筛选和临床研究的年龄，相关的黄斑变性。潜在用户是眼科和验光社区的从业人员。