MIMICS OF HELIX TURN HELIX PEPTIDES

螺旋转角螺旋肽的模拟物

基本信息

  • 批准号:
    6180628
  • 负责人:
  • 金额:
    $ 4.37万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-05-01 至 2000-08-09
  • 项目状态:
    已结题

项目摘要

DESCRIPTION: The goal of this project is to mimic the helix-turn-helix (HTH) motif of the POU Oct-1 homeodomain and the 434 Cro repressor by constraining the native conformation of this small portion of the protein. The helix-turn-helix motif is found in several DNA-binding proteins that have known 3-dimensional structures. Such chimeric molecules can serve a two-fold health-related purpose. First, the design and biochemical evaluation of the chimeric molecules will explore fundamental aspects of protein folding and protein-DNA interactions. Second, each of the mimics described could serve as biochemical probes of developmental processes. POU homeodomains are transcription factors for human growth hormone, histones, snRNAs, immunoglobulin, herpes simplex virus and other medically important genes. The PI proposes to constrain the conformation of peptides corresponding to the HTH motif via covalent bonds between buried hydrophobic side-chains. Two approaches to this unusual type of side-chain linkage will be taken: 1) aryl linkage and 2) alkyl linkage. The templates are designed to stabilize the conformation of the flexible turn. The designs feature stable organic side-chains which will favor protein folding by burial of the additional hydrophobic moiety. Each template will be synthesized stereospecifically from amino acid starting materials and incorporated by solid-phase synthesis into a peptide corresponding to the sequence of the structurally well-characterized Cro repressor and POU Oct-1 homeodomain. Peptide engineering of the alpha-helices will introduce salt bridges and residues with high helix propensities to improve the helicity of attached peptides. A helix-to-helix crosslink will be introduced to improve the tertiary structure stability. The HTH mimics will be characterized chemically (HPLC, MS, NMR), structurally (CD, NMR and/or x-ray crystallography) and functionally (DNA-binding affinity) to ascertain the success of the mimics.
描述:这个项目的目标是模拟螺旋-螺旋-螺旋

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

FELICIA A ETZKORN其他文献

FELICIA A ETZKORN的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('FELICIA A ETZKORN', 18)}}的其他基金

Liquid Chromatograph-Tandem Mass Spectrometer
液相色谱-串联质谱仪
  • 批准号:
    6441317
  • 财政年份:
    2002
  • 资助金额:
    $ 4.37万
  • 项目类别:
Designed Inhibitors of Pin1 in Mitosis
有丝分裂中 Pin1 的设计抑制剂
  • 批准号:
    7270460
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
Designed Inhibitors of Pin1 in Mitosis
有丝分裂中 Pin1 的设计抑制剂
  • 批准号:
    7477724
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
Designed Inhibitors of Pin1 in Mitosis
有丝分裂中 Pin1 的设计抑制剂
  • 批准号:
    7031406
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
Designed Inhibitors of Pin1 in Mitosis
有丝分裂中 Pin1 的设计抑制剂
  • 批准号:
    7667517
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
Designed Inhibitors of Pin1 in Mitosis
有丝分裂中 Pin1 的设计抑制剂
  • 批准号:
    7126863
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
PIN1 在有丝分裂中与设计抑制剂的作用
  • 批准号:
    6335974
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
PIN1 在有丝分裂中与设计抑制剂的作用
  • 批准号:
    6387319
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
THE ROLE OF PIN1 IN MITOSIS WITH DESIGNED INHIBITORS
PIN1 在有丝分裂中与设计抑制剂的作用
  • 批准号:
    6526115
  • 财政年份:
    2000
  • 资助金额:
    $ 4.37万
  • 项目类别:
MIMICS OF HELIX TURN HELIX PEPTIDES
螺旋转角螺旋肽的模拟物
  • 批准号:
    6436988
  • 财政年份:
    1997
  • 资助金额:
    $ 4.37万
  • 项目类别:

相似海外基金

Non-canonical chimeric proteins generated during Adenovirus infection
腺病毒感染期间产生的非典型嵌合蛋白
  • 批准号:
    10448505
  • 财政年份:
    2021
  • 资助金额:
    $ 4.37万
  • 项目类别:
Non-canonical chimeric proteins generated during Adenovirus infection
腺病毒感染期间产生的非典型嵌合蛋白
  • 批准号:
    10312411
  • 财政年份:
    2021
  • 资助金额:
    $ 4.37万
  • 项目类别:
Increasing efficiency in formation of chimeric proteins
提高嵌合蛋白形成的效率
  • 批准号:
    561998-2021
  • 财政年份:
    2021
  • 资助金额:
    $ 4.37万
  • 项目类别:
    University Undergraduate Student Research Awards
Decoration and Dimerization of Chimeric Proteins Mediated by Coiled-Coil Interactions
卷曲螺旋相互作用介导的嵌合蛋白的修饰和二聚化
  • 批准号:
    537306-2018
  • 财政年份:
    2019
  • 资助金额:
    $ 4.37万
  • 项目类别:
    Collaborative Research and Development Grants
Exploring the therapeutic potential of chimeric proteins
探索嵌合蛋白的治疗潜力
  • 批准号:
    1947736
  • 财政年份:
    2017
  • 资助金额:
    $ 4.37万
  • 项目类别:
    Studentship
TARGETING PROTEIN INTERACTIONS AND DESIGNING CHIMERIC PROTEINS
靶向蛋白质相互作用并设计嵌合蛋白质
  • 批准号:
    8364271
  • 财政年份:
    2011
  • 资助金额:
    $ 4.37万
  • 项目类别:
Therapeutic peanut allergen Fc gamma chimeric proteins to treat peanut allergy
用于治疗花生过敏的治疗性花生过敏原 Fc γ 嵌合蛋白
  • 批准号:
    8444422
  • 财政年份:
    2010
  • 资助金额:
    $ 4.37万
  • 项目类别:
Cat allergen-human Fc-gamma1 chimeric proteins to treat cat allergy
猫过敏原-人Fc-gamma1嵌合蛋白治疗猫过敏
  • 批准号:
    7907314
  • 财政年份:
    2010
  • 资助金额:
    $ 4.37万
  • 项目类别:
TARGETING PROTEIN INTERACTIONS AND DESIGNING CHIMERIC PROTEINS
靶向蛋白质相互作用并设计嵌合蛋白质
  • 批准号:
    8171849
  • 财政年份:
    2010
  • 资助金额:
    $ 4.37万
  • 项目类别:
Therapeutic peanut allergen Fc gamma chimeric proteins to treat peanut allergy
用于治疗花生过敏的治疗性花生过敏原 Fc γ 嵌合蛋白
  • 批准号:
    8313432
  • 财政年份:
    2010
  • 资助金额:
    $ 4.37万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了