Liquid Chromatograph-Tandem Mass Spectrometer

液相色谱-串联质谱仪

基本信息

批准号：
6441317
负责人：
FELICIA A ETZKORN
金额：
$ 40.72万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至 2003-05-31
项目状态：
已结题

项目摘要

Funding is requested for the acquisition of a liquid chromatography- tandem mass spectrometer (LC-MS-MS). Major users are from the departments of Chemistry (3), Biochemistry (2), and Biology (2), representing 9 NIH-funded projects. Minor users not funded by the NIH are from Chemistry (5), Biology (1) Biochemistry (2), and Entomology (2). Of the minor projects, two grant proposals are pending with the NIH and are likely to become major users. The LC-MS-MS is required for modern chemical biology, a rising field in biomedical research. A triple quadrupole MS-MS with an integrated capillary LC fulfills the requirements for the broad range of projects proposed to use the instrument, from natural product isolation, quantitation in metabolism studies and combinatorial chemistry for small molecules to sequencing of peptides and phosphoproteins in the macromolecular area. This instrument would have the unique capabilities of a triple quadrupole MS-MS at Virginia Tech. Prof. Castagnoli will discover and characterize neuroprotective and neurotoxic agents. Prof. Etzkorn will design and synthesize inhibitors of cell-cycle enzymes as potential anti- cancer agents. Prof. Kingston will elucidate the structures of natural products, design and synthesize derivatives of paclitaxel and study the mechanism of paclitaxel binding to tubulin and microtubules. Prof. Popham will characterize bacterial cell wall synthetic enzymes and analyze changes in wall structure. Prof. Carlier will design and synthesize bi- and multivalent probes of the GABA(A) receptor, and will use acetylcholinesterase to catalyze non-native chemical reactions. Prof. Taylor will demonstrate that supercritical fluid chromatography is a viable interface for mass spectral identification/quantification of drug- like materials. Prof. White will establish coenzyme biosynthetic intermediates in the Archaea. The instrument will be housed in a shared instrumentation laboratory located in the Chemistry Department and operated as a user-accessible, operator-maintained facility. The P.I., Felicia Etzkorn, in conjunction with Geno Iannaccone, Director of Analytical Services, is responsible for purchase, scientific and administrative oversight of the LC-MS-MS. User training, operation, maintenance and repair of the instrument will be the responsibility of the current mass spectrometrist, Ms. Anne Campbell.

要求获得液相色谱 - 串联质谱仪（LC-MS-MS）的资金。主要用户来自化学部（3），生物化学（2）和生物学（2），代表9个NIH资助的项目。未由NIH资助的未成年人来自化学（5），生物学（1）生物化学（2）和昆虫学（2）。在次要项目中，两项赠款提案正在与NIH一起审理，并可能成为主要用户。 LC-MS-MS是现代化学生物学所必需的，这是生物医学研究的上升领域。具有集成毛细管LC的三倍四极杆MS-MS满足了对使用该仪器的广泛项目的要求，从天然产物隔离，代谢研究中的定量和对小分子的组合化学到对大分子的测序和肽蛋白在大分子区域的测序。该乐器将具有弗吉尼亚理工学院三倍四倍的MS-MS的独特功能。 Castagnoli教授将发现并表征神经保护剂和神经毒性剂。 Etzkorn教授将设计和合成细胞周期酶的抑制剂作为潜在的抗癌药物。金斯敦教授将阐明紫杉醇的天然产物，设计和合成的衍生物的结构，并研究紫杉醇与微管蛋白和微管的机理。 Popham教授将表征细菌细胞壁合成酶，并分析壁结构的变化。 Carlier教授将设计和合成GABA（A）受体的双价和多价探针，并将使用乙酰胆碱酯酶催化非本地化学反应。泰勒教授将证明超临界流体色谱法是用于质谱/定量类似材料的可行界面。怀特教授将在古细菌中建立辅酶生物合成中间体。该仪器将安置在化学部门位于化学部门的共享仪器实验室中，并作为用户访问，操作员维护的设施运行。 P.I. Felicia Etzkorn与分析服务总监Geno Iannaccone一起负责购买，科学和行政的LC-MS-MS监督。该工具的用户培训，操作，维护和维修将负责当前的质谱师Anne Campbell女士的责任。