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Liquid Chromatograph-Tandem Mass Spectrometer

液相色谱-串联质谱仪

基本信息

批准号：
6441317
负责人：
FELICIA A ETZKORN
金额：
$ 40.72万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至 2003-05-31
项目状态：
已结题

项目摘要

Funding is requested for the acquisition of a liquid chromatography- tandem mass spectrometer (LC-MS-MS). Major users are from the departments of Chemistry (3), Biochemistry (2), and Biology (2), representing 9 NIH-funded projects. Minor users not funded by the NIH are from Chemistry (5), Biology (1) Biochemistry (2), and Entomology (2). Of the minor projects, two grant proposals are pending with the NIH and are likely to become major users. The LC-MS-MS is required for modern chemical biology, a rising field in biomedical research. A triple quadrupole MS-MS with an integrated capillary LC fulfills the requirements for the broad range of projects proposed to use the instrument, from natural product isolation, quantitation in metabolism studies and combinatorial chemistry for small molecules to sequencing of peptides and phosphoproteins in the macromolecular area. This instrument would have the unique capabilities of a triple quadrupole MS-MS at Virginia Tech. Prof. Castagnoli will discover and characterize neuroprotective and neurotoxic agents. Prof. Etzkorn will design and synthesize inhibitors of cell-cycle enzymes as potential anti- cancer agents. Prof. Kingston will elucidate the structures of natural products, design and synthesize derivatives of paclitaxel and study the mechanism of paclitaxel binding to tubulin and microtubules. Prof. Popham will characterize bacterial cell wall synthetic enzymes and analyze changes in wall structure. Prof. Carlier will design and synthesize bi- and multivalent probes of the GABA(A) receptor, and will use acetylcholinesterase to catalyze non-native chemical reactions. Prof. Taylor will demonstrate that supercritical fluid chromatography is a viable interface for mass spectral identification/quantification of drug- like materials. Prof. White will establish coenzyme biosynthetic intermediates in the Archaea. The instrument will be housed in a shared instrumentation laboratory located in the Chemistry Department and operated as a user-accessible, operator-maintained facility. The P.I., Felicia Etzkorn, in conjunction with Geno Iannaccone, Director of Analytical Services, is responsible for purchase, scientific and administrative oversight of the LC-MS-MS. User training, operation, maintenance and repair of the instrument will be the responsibility of the current mass spectrometrist, Ms. Anne Campbell.

申请资金用于购置一台液相色谱-串联质谱仪。主要用户来自化学（3），生物化学（2）和生物学（2），代表9个NIH资助的项目。不受NIH资助的少数用户来自化学（5），生物学（1），生物化学（2）和昆虫学（2）。在这些小项目中，有两个赠款提案正在等待NIH批准，很可能成为主要用户。LC-MS-MS是现代化学生物学所必需的，这是生物医学研究中的一个新兴领域。一个三重四极杆MS-MS与集成毛细管LC满足广泛的项目提出使用该仪器的要求，从天然产物的分离，定量代谢研究和组合化学的小分子肽和磷蛋白在大分子领域的测序。该仪器将具有弗吉尼亚理工大学的三重四极杆MS-MS的独特能力。Castagnoli教授将发现和表征神经保护和神经毒性药物。Etzkorn教授将设计和合成细胞周期酶抑制剂作为潜在的抗癌药物。金斯顿教授将阐明天然产物的结构，设计和合成紫杉醇衍生物，并研究紫杉醇与微管蛋白和微管结合的机制。Popham教授将表征细菌细胞壁合成酶并分析细胞壁结构的变化。Carlier教授将设计和合成GABA（A）受体的双价和多价探针，并将使用乙酰胆碱酯酶催化非天然化学反应。Taylor教授将证明超临界流体色谱法是一种可行的质谱鉴定/定量药物样物质的接口。白色教授将建立辅酶生物合成中间体的酶。仪器将放置在化学系的共用仪器实验室中，并作为用户可访问、操作员维护的设施运行。私家侦探，Felicia Etzkorn与分析服务总监Geno Iannaccone一起负责LC-MS-MS的采购、科学和管理监督。仪器的用户培训、操作、维护和维修将由现任质谱师Anne坎贝尔女士负责。