ASSEMBLY OF THE SPORE COAT OF B SUBTILIS

枯草芽孢杆菌孢子衣的组装

• 批准号: 6180880
• 负责人: Adam Driks
• 金额: $ 11.07万
• 依托单位: LOYOLA UNIVERSITY CHICAGO
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6180880
• 关键词: Bacillus subtilis; SDS polyacrylamide gel electrophoresis; bacterial genetics; bacterial proteins; electron microscopy; gene deletion mutation; gene expression; genetic mapping; genetic strain; immunoelectron microscopy; intermolecular interaction; intracellular; laboratory rabbit; life cycle; mutant; organelles; polymerase chain reaction; protein biosynthesis; protein structure function; site directed mutagenesis; spores; western blottings

Without the capacity to assemble into specific structures at particular sites within a cell, proteins would not be able to build the complex organelles that carry out cell division, chemotaxis, photosynthesis or chromosome segregation. Disruption or reprogramming of processes of assembly and localization have disastrous consequences for the cell and thus the health of the organism. For example, enteropathogenic E. coli induce the rearrangement of the cytoskeleton of cells in the human gut, which is followed by vomiting and diarrhea. Listeria monocytogenes also alters the assembly of host cytoskeletal proteins in the course of infection. This organism recruits actin from the host-cell cytoplasm to form a comet-like tail which it uses to reach the host cell surface and then to infect adjacent cells. The formation of the structurally complex, multilayered coat that surrounds the B. subtilis spore provides one of the most powerful systems for the study of subcellular localization and assembly because B. subtilis is extremely amenable to genetic manipulation and morphological analysis. As a result of the application of these tools, the process of sporulation is understood in outline and studies of the assembly of spore structures have become feasible. Our long term goals are to elucidate the mechanisms that guide spore coat assembly to occur at the particular site of the spore surface and that control the formation of the coat layers. Our specific goals are to understand how two critical players in spore coat assembly, the proteins CotE and SpoIVA, control the formation of the spore coat and to identify additional proteins that are involved in spore coat morphogenesis. We will determine which regions of SpoIVA and CotE are important in coat assembly by a mutational analysis of their genes, and we will use both genetic and biochemical approaches to identify additional morphogenetic proteins. Ultimately, we hope to understand not only how the B. subtilis spore coat is formed but also to gain insight into organelle biosynthesis in a wide variety of organisms.

没有能力在特定的时间组装成特定的结构， 蛋白质就不能在细胞内构建复合物 进行细胞分裂、趋化性、光合作用或 染色体分离中断或重新编程的过程 组装和定位对细胞具有灾难性的后果， 从而影响生物体的健康。例如，肠致病性E.杆菌 诱导人肠道细胞骨架的重排， 然后是呕吐和腹泻单核细胞增生李斯特菌还 改变宿主细胞骨架蛋白的组装过程中， 感染这种生物从宿主细胞质中募集肌动蛋白， 形成一个彗星状的尾巴，它用来到达宿主细胞表面， 然后感染邻近的细胞。 结构复杂的多层涂层的形成， 围绕着B。枯草芽孢杆菌孢子提供了一个最强大的系统， 用于亚细胞定位和组装的研究，因为B. 枯草芽孢杆菌是非常适合遗传操作和形态 分析.由于这些工具的应用， 孢子形成是了解在大纲和研究的组装孢子 结构变得可行。我们的长期目标是阐明 引导孢子衣在特定部位组装的机制 控制着孢子表面的结构，并控制着被膜层的形成。 我们的具体目标是了解孢子中的两个关键角色 外壳组装，蛋白质CotE和SpoIVA，控制着外壳的形成。 以鉴定孢子中涉及其它蛋白质 被毛形态发生我们将确定SpoIVA和CotE的哪些区域 通过对它们基因的突变分析， 我们将用遗传学和生物化学的方法来鉴定 其他形态发生蛋白。最终，我们希望了解的不是 只是B.枯草芽孢杆菌孢子衣的形成，但也获得洞察力 转化为各种生物体中的细胞器生物合成。

项目成果

Characterization of a Bacillus anthracis spore coat-surface protein that influences coat-surface morphology.

• DOI: 10.1111/j.1574-6968.2008.01380.x
• 发表时间: 2008-12
• 期刊: FEMS microbiology letters
• 影响因子: 2.1