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ASSEMBLY OF THE SPORE COAT OF B SUBTILIS

枯草芽孢杆菌孢子衣的组装

基本信息

批准号：
6180880
负责人：
Adam Driks
金额：
$ 11.07万
依托单位：
LOYOLA UNIVERSITY CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-05-01 至 2002-04-30
项目状态：
已结题

项目摘要

Without the capacity to assemble into specific structures at particular sites within a cell, proteins would not be able to build the complex organelles that carry out cell division, chemotaxis, photosynthesis or chromosome segregation. Disruption or reprogramming of processes of assembly and localization have disastrous consequences for the cell and thus the health of the organism. For example, enteropathogenic E. coli induce the rearrangement of the cytoskeleton of cells in the human gut, which is followed by vomiting and diarrhea. Listeria monocytogenes also alters the assembly of host cytoskeletal proteins in the course of infection. This organism recruits actin from the host-cell cytoplasm to form a comet-like tail which it uses to reach the host cell surface and then to infect adjacent cells. The formation of the structurally complex, multilayered coat that surrounds the B. subtilis spore provides one of the most powerful systems for the study of subcellular localization and assembly because B. subtilis is extremely amenable to genetic manipulation and morphological analysis. As a result of the application of these tools, the process of sporulation is understood in outline and studies of the assembly of spore structures have become feasible. Our long term goals are to elucidate the mechanisms that guide spore coat assembly to occur at the particular site of the spore surface and that control the formation of the coat layers. Our specific goals are to understand how two critical players in spore coat assembly, the proteins CotE and SpoIVA, control the formation of the spore coat and to identify additional proteins that are involved in spore coat morphogenesis. We will determine which regions of SpoIVA and CotE are important in coat assembly by a mutational analysis of their genes, and we will use both genetic and biochemical approaches to identify additional morphogenetic proteins. Ultimately, we hope to understand not only how the B. subtilis spore coat is formed but also to gain insight into organelle biosynthesis in a wide variety of organisms.

没有能力在特定情况下组装成特定结构细胞内的位点，蛋白质将无法构建复合物进行细胞分裂、趋化性、光合作用或染色体分离。进程的中断或重新编程组装和定位会给细胞带来灾难性的后果从而保证机体的健康。例如，致病性大肠杆菌诱导人体肠道细胞骨架的重排，接下来是呕吐和腹泻。单核细胞增生李斯特氏菌也改变宿主细胞骨架蛋白的组装感染。该生物体从宿主细胞的细胞质中招募肌动蛋白形成彗星状的尾巴，用于到达宿主细胞表面并然后感染邻近的细胞。结构复杂的多层涂层的形成围绕枯草芽孢杆菌孢子提供了最强大的系统之一用于亚细胞定位和组装的研究，因为 B. 枯草芽孢杆菌非常适合遗传操作和形态学研究分析。由于应用了这些工具，该过程孢子形成的概述和孢子组装的研究结构已变得可行。我们的长期目标是阐明引导孢子衣组装在特定位点发生的机制孢子表面并控制包衣层的形成。我们的具体目标是了解《孢子》中的两个关键角色如何外壳组装，蛋白质 CotE 和 SpoIVA，控制着孢子外壳并鉴定孢子中涉及的其他蛋白质外套形态发生。我们将确定 SpoIVA 和 CotE 的哪些区域通过对其基因的突变分析，它们在外套组装中很重要，我们将使用遗传和生化方法来识别额外的形态发生蛋白。最终，我们希望理解不不仅了解枯草芽孢杆菌孢子衣的形成方式，还获得深入了解进入多种生物体的细胞器生物合成。