IDENTIFICATION OF B. ANTRACIS SPORE-SURFACE PROTEINS

B.ANTRACIS 孢子表面蛋白的鉴定

• 批准号: 6562224
• 负责人: Adam Driks
• 金额: $ 22.2万
• 依托单位: LOYOLA UNIVERSITY CHICAGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6562224
• 关键词: Bacillus anthracis; Bacillus subtilis; anthrax; bacterial DNA; bacterial antigens; bacterial proteins; bioterrorism /chemical warfare; chimeric proteins; flow cytometry; genetic library; open reading frames; proteomics; recombinant DNA; spores

DESCRIPTION (provided by applicant): As current events dramatically indicate, reliable accurate and portable Bacillus anthracis spore detection systems are critical for national defense and civilian health. In an effort to meet this need, several candidate technologies are under examination by a number of federal agencies. Detectors using the outermost proteins of the spore coat as signature molecules for identification would represent a particularly compelling technology. This approach is not currently feasible, as the identities of the B. anthracis surface proteins are unknown. However, based on the completed B. anthracis genome sequence and extensive analysis of the related organism B. subtilis, we can now identify many spore-surface protein candidates. In addition, a variety of approaches to identify these proteins de novo are available. Therefore, we propose using a combination of biochemical and genetic methods to test candidate proteins for a surface location as well as to identify these proteins directly. Our approaches take advantage of proteomic analysis of the B. anthracis and B. subtilis coats ongoing in our laboratory, as well as the available genome sequences of the two organisms. Ultimately, this work will enable development of a new class of small, robust versatile B. anthracis spore detectors.

描述（由申请人提供）：正如当前事件戏剧性地表明的那样，可靠、准确和便携式炭疽芽孢杆菌孢子检测系统对于国防和平民健康至关重要。 为了满足这一需求，若干联邦机构正在审查若干候选技术。 利用孢子外壳最外层的蛋白质作为识别特征分子的探测器将代表一种特别引人注目的技术。 这种方法目前是不可行的，因为身份的B。炭疽表面蛋白是未知的。 但是，根据已完成的B。炭疽菌基因组序列和相关生物B的广泛分析。枯草芽孢杆菌，我们现在可以确定许多孢子表面蛋白的候选者。 此外，有多种方法可以从头鉴定这些蛋白质。 因此，我们建议使用生物化学和遗传学方法的组合来测试候选蛋白的表面位置，以及直接识别这些蛋白。 我们的方法利用蛋白质组学分析的B。炭疽菌和B. B.枯草芽孢杆菌的外套正在我们的实验室，以及可用的基因组序列的两种生物。 最终，这项工作将能够开发出一种新的小型，强大的多功能B。炭疽孢子探测器