FUNCTIONAL SIGNIFICANCE OF HIPPOCAMPAL SPROUTING

海马萌芽的功能意义

• 批准号: 6223610
• 负责人: JULIO J RAMIREZ
• 金额: $ 14.7万
• 依托单位: DAVIDSON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6223610
• 关键词: brain injury; entorhinal cortex; hippocampus; laboratory rat; long term potentiation; memory; neural facilitation; neuronal guidance; spreading cortical depression

DESCRIPTION (Adapted from the Investigator's Abstract) Neuronal sprouting has been observed post-mortem in the hippocampal formation of patients suffering from Alzheimer's disease. The possibility exists that the reorganization of hippocampal circuitry may: 1) contribute to the behavioral symptoms associated with the disease, or 2) compensate for the loss of hippocampal neurons projecting to the hippocampus. Unfortunately, little is presently known about the behavioral significance of sprouting in the central nervous system (CNS). The purpose of this small-scale investigation is to evaluate the functional significance of sprouting in the dentate gyrus of the hippocampal formation after unilateral lesions of the entorhinal cortex in rats. This study will focus on the synaptic efficacy of a proliferated homologous input (i.e., the crossed temporodentate projection - CTD) after a one stage or progressive EC lesion. Progressive EC lesions are known to accelerate septodentate, commissural/associational, and CTD sprouting to the dentate gyrus; however, the effects of the progressive lesions on the ability of the CTD to support long term potentiation and paired-pulse potentiation, two measures of synaptic efficacy, are unknown. The sprouted CTD pathway is of special interest because: 1) it emerges from the same cell layer in the contralateral homologue as the normal ipsilateral entorhinal input to the dentate gyrus; and, 2) it has been implicated in the recovery of spatial memory after unilateral entorhinal injury. Particularly noteworthy, progressive lesions of the entorhinal area have been shown to spare spatial memory from the deficits typically associated with entorhinal injury. This electrophysiological analysis will lay the foundation for future work assessing the functional significance of CNS sprouting.

描述（改编自研究者摘要） 在死后海马结构中观察到神经元发芽 老年痴呆症患者的研究。可能性存在该 海马电路的重组可能：1）有助于行为 与疾病相关的症状，或2）补偿损失 海马神经元投射到海马。不幸的是， 目前已知的行为意义的萌芽，在中央 神经系统（CNS）。这次小规模调查的目的是 评估脑齿状回发芽的功能意义， 内嗅皮层单侧损伤后海马结构的变化 大鼠这项研究将集中在一个增殖的突触功效， 同源输入（即，交叉颞齿状突（CTD）， 1期或进行性EC病变。已知进行性EC病变 加速隔齿、连合/联合和CTD发芽到 齿状回;然而，进行性病变对能力的影响 的CTD支持长时程增强和双脉冲增强，两个 突触功效的测量尚不清楚。发芽的CTD途径是 特别感兴趣，因为：1）它出现在同一个细胞层中， 对侧同源物作为正常同侧内嗅输入到 齿状回; 2）它与空间记忆的恢复有关 单侧内嗅损伤后特别值得注意的，进步的 内嗅区的损伤已经被证明可以使空间记忆从 通常与内嗅损伤有关的缺陷。这种电生理 分析将为今后的工作奠定基础， CNS发芽的意义