LIPID CONTROL IN THE REMOVAL OF DYING BRAIN TUMOR CELLS

描述：细胞凋亡(或程序性细胞死亡)并不完全 据了解，申请者的研究小组已经表明，恶性肿瘤中的细胞凋亡 神经元型HN2-5细胞系与饱和脂肪增加有关 酸性磷脂，增加了膜的硬度，并随着表达 5-羟色胺1A受体(5-HT1A-R)。申请人假定一名 膜脂组成的变化抑制了膜结合酶，如 帮助定位的氨基磷脂转位酶(APTL) 磷脂酰丝氨酸(PS)到Inne膜小叶。收入的再分配 在细胞凋亡过程中，PS到外叶似乎是一个主要的信号 触发小胶质细胞对凋亡细胞的吞噬作用。的总体目标 这个程序是1)了解膜脂成分的变化 在HN2-5细胞中发生的凋亡和2)确定这些变化如何 影响TH小胶质细胞吞噬凋亡细胞。申请人 提出的研究要么模仿磷脂膜的变化，要么 以受控的方式复制它们。他还会尝试引诱一些 在维持肿瘤存活的同时，磷脂的变化 细胞。本项目将帮助您了解一些 在细胞凋亡过程中发生的细胞膜改变。

DESCRIPTION: Apoptosis (or programmed cell death) is not completely understood The applicant's group has shown that apoptosis in the malignant neuronal HN2-5 cell line is associated with an increase in saturated fatty acid- phospholipid which increase membrane rigidity and with the expression of serotonin 1A receptor (5-HT1A -R). The applicant postulates that a change in membrane lipid composition inhibits such membrane-bound enzymes as aminophospholipid translocase (APTL) which helps to localize phosphatidylserine (PS) to the inne membrane leaflet. The redistribution of PS to the outer leaflet during apoptosis appears to be a dominant signal to trigger phagocytosis of apoptotic cells by microglia. The overall goals of this program are 1) to understand wha changes in membrane lipid composition occur in HN2-5 cells during apoptosis an 2) to determine how these changes affect phagocytosis of apoptotic cells by th microglia. The applicant proposes studies that will either mimic the phospholipid membrane changes or reproduce them in a controlled manner. He wil also attempt to induce some of the phospholipid changes while maintaining the viability of the tumor cells. This project will help provide an understanding of some of the membrane alterations that occur during apoptosis.