GENETIC ANALYSIS OF ZEBRAFISH EMBRYO DEVELOPMENT

斑马鱼胚胎发育的遗传分析

• 批准号: 6162609
• 负责人: P P LIU
• 金额: --
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6162609
• 关键词: animal genetic material tag; developmental genetics; gene expression; genetic library; genetic promoter element; genetic regulation; green fluorescent proteins; in situ hybridization; microinjections; molecular cloning; vertebrate embryology; zebrafish

We will use zebrafish system to study the genetic control of vertebrate embryogenesis. We are especially interested in the genes playing important roles in human disease pathogenesis. For each gene of interest, zebrafish cDNA libraries will be screened and the zebrafish homolog identified. The cDNA clones will then be used as probes for RNA in situ hybridization to analyze the expression pattern during fish embryogenesis. Promoter-green fluorescent protein constructs will allow us to study gene expression in live embryos. The functional role of individual genes during embryogenesis will be assessed by microinjection of DNA, RNA, and oligo constructs into embryos. Finally, the potential involvement of these genes in fish mutants with defects in embryogenesis will be analyzed using genome analysis tools.

我们将利用斑马鱼系统来研究脊椎动物的遗传控制 胚胎发生。我们对基因发挥的作用特别感兴趣 在人类疾病发病机制中的重要作用。对于每个感兴趣的基因， 斑马鱼的cDNA文库将被筛选，斑马鱼的同源物 确认身份。然后，这些克隆将被用作原位RNA的探针 杂交技术在FISH中的表达模式分析 胚胎发生。启动子-绿色荧光蛋白构建将允许 美国将研究活胚胎中的基因表达。的职能作用 胚胎发育过程中的单个基因将通过显微注射进行评估。 DNA、RNA和寡核苷酸构建成胚胎。最后，潜在的 这些基因与鱼类胚胎发育缺陷突变体的关系 将使用基因组分析工具进行分析。