DESIGN AND CONSTRUCTION OF HUMAN ARTIFICIAL CHROMOSOME VECTORS

人类人工染色体载体的设计和构建

• 批准号: 5203422
• 负责人: P P LIU
• 金额: --
• 依托单位: NATIONAL HUMAN GENOME RESEARCH INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5203422
• 关键词: biotechnology; chromosomes; fusion gene; gene expression; gene therapy; genetic manipulation; regulatory gene; technology /technique development; transfection /expression vector

In collaboration with Dr. Melissa Rosenfeld, a colleague in the Laboratory of Gene Transfer, we are constructing human artificial chromosomes (HAC) that may provide the ideal gene delivery system. The HAC vector would not integrate into endogenous chromosomes, eliminating the possibility of insertional mutagenesis. It can, in theory, accept unlimited sizes of DNA, so large genes and long stretches of surrounding regulatory sequences can be transferred. Design features of the vectors such as centromeres, telomeres, and replication origins should facilitate the stable maintenance and correct segregation of the vector in human cells.

在与Melissa Rosenfeld博士的合作中， 基因转移实验室，我们正在构建人类人工 染色体（HAC），可以提供理想的基因传递系统。的 HAC载体不会整合到内源染色体中，从而消除了 插入突变的可能性。理论上，它可以接受 无限大小的DNA，所以大的基因和长的周围环境 可以转移调节序列。载体的设计特点 如着丝粒、端粒和复制起点， 载体在人体内的稳定维持和正确分离 细胞